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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab73877? Please let us know so that we can cite the reference in this datasheet
ab73877 has been referenced in 2 publications.
- Cheng L et al. Elevated invasive potential of glioblastoma stem cells. Biochem Biophys Res Commun 406:643-8 (2011). WB; Human. PubMed: 21371437
- Li C et al. Synergistic Activation of Dopamine D1 and TrkB Receptors Mediate Gain Control of Synaptic Plasticity in the Basolateral Amygdala. PLoS One 6:e26065 (2011). IHC-FoFr; Rat. PubMed: 22022509
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP16 antibody (ab73877)
Immunohistochemical analysis of paraffin-embedded sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.
Immunohistochemistry (Frozen sections) - MMP16 antibody (ab73877)
Immunohistochemical analysis of frozen sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.
Western blot - MMP16 antibody (ab73877)
All lanes : Anti-MMP16 antibody (ab73877) at 1 µg/ml
Lane 1 : Whole cell lysates prepared from Human Hela cells.
Lane 2 : Whole cell lysates prepared from Human Jurkat cells.
Lane 3 : Whole cell lysates prepared from Human Colo320 cells.
Lysates/proteins at 50 µg per lane.
Secondary
HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size : 57 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MMP16 antibody (ab73877)
ICC/IF image of ab73877 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73877, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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