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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP16 antibody
See all MMP16 products (9) ...
Description
Rabbit polyclonal to MMP16
Tested applications
WB, IHC-P, IHC-Fr, ICC/IF, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide mapping at the C-terminal of human MMP16.
Positive control
Rat kindey tissue (frozen sections and paraffin-embedded sections), Hela cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab73877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 57 kDa.
IHC-P: Use a concentration of 0.5 - 1 µg/ml.
IHC-Fr: Use a concentration of 0.5 - 1 µg/ml.
ICC/IF: Use a concentration of 5 µg/ml
IHC-FoFr: Use at an assay dependent concentration. (PubMed: 22022509)
Target
Function
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
Tissue specificity
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Developmental stage
Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Cell membrane. Localized at the cell surface of melanoma cells and Secreted > extracellular space > extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells.
Target information above from: UniProt accessionP51512
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix metallopeptidase 16 membrane inserted antibody
- Matrix metalloproteinase 16 antibody
- Matrix metalloproteinase-16 antibody
see all
Database links
- Entrez Gene: 4325 Human
- Entrez Gene: 17389 Mouse
- Entrez Gene: 65205 Rat
- Omim: 602262 Human
- SwissProt: P51512 Human
- SwissProt: Q9WTR0 Mouse
- SwissProt: O35548 Rat
- Unigene: 492187 Human
see all
Anti-MMP16 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP16 antibody (ab73877)
Immunohistochemical analysis of paraffin-embedded sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.
Immunohistochemistry (Frozen sections) - MMP16 antibody (ab73877)
Immunohistochemical analysis of frozen sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.
Western blot - MMP16 antibody (ab73877)
All lanes : Anti-MMP16 antibody (ab73877) at 1 µg/ml
Lane 1 : Whole cell lysates prepared from Human Hela cells.
Lane 2 : Whole cell lysates prepared from Human Jurkat cells.
Lane 3 : Whole cell lysates prepared from Human Colo320 cells.
Lysates/proteins at 50 µg per lane.
Secondary
HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size : 57 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MMP16 antibody (ab73877)
ICC/IF image of ab73877 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73877, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-MMP16 antibody (ab73877)
This product has been referenced in:
- Cheng Let al. Elevated invasive potential of glioblastoma stem cells. Biochem Biophys Res Commun 406:643-8 (2011). WB; Human.Read more (PubMed: 21371437) »
- Li Cet al. Synergistic Activation of Dopamine D1 and TrkB Receptors Mediate Gain Control of Synaptic Plasticity in the Basolateral Amygdala. PLoS One 6:e26065 (2011). IHC-FoFr; Rat.Read more (PubMed: 22022509) »
See all 2 publications for this product
Publishing research using ab73877? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"