Anti-MMP16 antibody (ab73877)
Key features and details
- Rabbit polyclonal to MMP16
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MMP16 antibody
See all MMP16 primary antibodies -
Description
Rabbit polyclonal to MMP16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
A synthetic peptide mapping at the C-terminal of human MMP16.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
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IHC-Fr |
Use a concentration of 0.5 - 1 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 57 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. |
IHC-Fr
Use a concentration of 0.5 - 1 µg/ml. |
Target
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Function
Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells. -
Tissue specificity
Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Developmental stage
Expressed in tissues undergoing reconstruction. Present in fetal tissues, especially in brain. Expression seems to decline with advanced development. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Cell membrane. Localized at the cell surface of melanoma cells and Secreted > extracellular space > extracellular matrix. Cell surface. Localized at the cell surface of melanoma cells. - Information by UniProt
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Database links
- Entrez Gene: 4325 Human
- Entrez Gene: 65205 Rat
- Omim: 602262 Human
- SwissProt: P51512 Human
- SwissProt: O35548 Rat
- Unigene: 492187 Human
- Unigene: 546267 Human
- Unigene: 208361 Rat
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Alternative names
- C8orf57 antibody
- DKFZp761D112 antibody
- Matrix metalloproteinase 16 antibody
see all
Images
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All lanes : Anti-MMP16 antibody (ab73877) at 1 µg/ml
Lane 1 : Whole cell lysates prepared from Human Hela cells.
Lane 2 : Whole cell lysates prepared from Human Jurkat cells.
Lane 3 : Whole cell lysates prepared from Human Colo320 cells.
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution
Predicted band size: 57 kDa -
Immunohistochemical analysis of paraffin-embedded sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.
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Immunohistochemical analysis of frozen sections Rat kindey using MMP16 antibody ab73877 at 0.5 - 1 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab73877 has been referenced in 13 publications.
- Schniers BK et al. PEPT1 is essential for the growth of pancreatic cancer cells: a viable drug target. Biochem J 478:3757-3774 (2021). PubMed: 34569600
- Zhou H & Chen Y CircRNA has_circ_0001806 promotes hepatocellular carcinoma progression via the miR-193a-5p/MMP16 pathway. Braz J Med Biol Res 54:e11459 (2021). PubMed: 34730679
- Zhang Y et al. Circular RNA hsa_circ_0002360 promotes non-small cell lung cancer progression through upregulating matrix metalloproteinase 16 and sponging multiple micorRNAs. Bioengineered 12:12767-12777 (2021). PubMed: 34747300
- Xu E et al. Exogenous HMGN2 inhibits the migration and invasion of osteosarcoma cell lines. Transl Cancer Res 9:1795-1805 (2020). PubMed: 35117527
- Zheng W et al. microRNA-26a Directly Targeting MMP14 and MMP16 Inhibits the Cancer Cell Proliferation, Migration and Invasion in Cutaneous Squamous Cell Carcinoma. Cancer Manag Res 12:7087-7095 (2020). PubMed: 32848463