![Western blot - MMP17 antibody [EP1270Y] (ab51075) image](#ab51075_1.bmp)
![Immunohistochemistry (Paraffin-embedded sections) - MMP17 antibody [EP1270Y] (ab51075) image](#ab51075_2.bmp)
![Immunocytochemistry/ Immunofluorescence - MMP17 antibody [EP1270Y] (ab51075) image](#ab51075_3.bmp)
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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP17 antibody [EP1270Y]
See all MMP17 products (7) ...
Description
Rabbit monoclonal [EP1270Y] to MMP17
Tested applications
WB, IHC-P, ICC/IF, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues on human MMP17.
Positive control
Mouse brain lysate, human lymphoma tissue or HeLa cells.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1270Y
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Detects a band of approximately 58 kDa (predicted molecular weight: 67 kDa).
IHC-P: 1/50 - 1/100.(We strongly recommend that customers perform an antigen retrieval step.)
ICC/IF: 1/50 - 1/100.
IHC-FoFr: Use at an assay dependent concentration. (PubMed: 22022509)
Application notes
Is unsuitable for ,Flow Cyt or IP.
Target
Function
Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.
Tissue specificity
Expressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Cell membrane. Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ9ULZ9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MTMMP 4 antibody
- Matrix metalloproteinase 17 antibody
- Matrix metalloproteinase 17 membrane inserted antibody
see all
Database links
- Entrez Gene: 4326 Human
- Entrez Gene: 23948 Mouse
- Entrez Gene: 288626 Rat
- Omim: 602285 Human
- SwissProt: Q9ULZ9 Human
- SwissProt: Q9R0S3 Mouse
- Unigene: 709245 Human
- Unigene: 42047 Mouse
see all
Anti-MMP17 antibody [EP1270Y] images:
Western blot - MMP17 antibody [EP1270Y] (ab51075)
![Western blot - MMP17 antibody [EP1270Y] (ab51075)](/ps/datasheet/Images/51/ab51075/ab51075_1.bmp)
Anti-MMP17 antibody [EP1270Y] (ab51075) at 1/1000 dilution + Mouse brain lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size : 67 kDa
Observed band size : 58 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - MMP17 antibody [EP1270Y] (ab51075)
![Immunohistochemistry (Paraffin-embedded sections) - MMP17 antibody [EP1270Y] (ab51075)](/ps/datasheet/Images/51/ab51075/ab51075_2.bmp)
Ab51075 (1:50) staining human MMP17 in human lymphoma tissue by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - MMP17 antibody [EP1270Y] (ab51075)
![Immunocytochemistry/ Immunofluorescence - MMP17 antibody [EP1270Y] (ab51075)](/ps/datasheet/Images/51/ab51075/ab51075_3.bmp)
Immunofluorescent staining of HeLa cells using ab51075 (1:50).
References for Anti-MMP17 antibody [EP1270Y] (ab51075)
This product has been referenced in:
- Sohail Aet al. Characterization of the Dimerization Interface of Membrane Type 4 (MT4)-Matrix Metalloproteinase. J Biol Chem 286:33178-89 (2011). WB; Mouse.Read more (PubMed: 21828052) »
- Li Cet al. Synergistic Activation of Dopamine D1 and TrkB Receptors Mediate Gain Control of Synaptic Plasticity in the Basolateral Amygdala. PLoS One 6:e26065 (2011). IHC-FoFr; Rat.Read more (PubMed: 22022509) »
See all 2 publications for this product
Publishing research using ab51075? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"