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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP17 antibody
See all MMP17 products (7) ...
Description
Rabbit polyclonal to MMP17
Specificity
Ab39708 binds to MMP17, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9,etc.). Also recognises activation/breakdown products. This antibody can recognize native or reduced MMP17. MMP17 has been reported to be elevated in several tumor cell lines, and is constituitively produced by some normal cell lines. Treatment of cells with Concanavolin A or the phorbol ester TPA stimulates production of MMP17 in some cell types, and the enzyme can be recovered in cell lysates. Shed forms of MMP17 have also been reported.
Tested applications
WB, IP, Flow Cyt, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide of Human MMP17. The antibody is made to the aminoterminal end of furin cleaved MMP17.
(Peptide available as ab43115.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - MMP17 antibody (ab39708)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Detects a band of approximately 63 (pro) , 65 (active) kDa (predicted molecular weight: 67 kDa).(A recommended starting concentration for Western blots is 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for non-human samples.)
IP: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
Target
Function
Endopeptidase that degrades various components of the extracellular matrix, such as fibrin. May be involved in the activation of membrane-bound precursors of growth factors or inflammatory mediators, such as tumor necrosis factor-alpha. May also be involved in tumoral process. Not obvious if able to proteolytically activate progelatinase A. Does not hydrolyze collagen types I, II, III, IV and V, gelatin, fibronectin, laminin, decorin nor alpha1-antitrypsin.
Tissue specificity
Expressed in brain, leukocytes, colon, ovary testis and breast cancer. Expressed also in many transformed and non-transformed cell types.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
The precursor is cleaved by a furin endopeptidase.
Cellular localization
Cell membrane. Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ9ULZ9
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- MTMMP 4 antibody
- Matrix Metalloproteinase 17 antibody
- Matrix Metalloproteinase 17 antibody
see all
Database links
- Entrez Gene: 4326 Human
- Omim: 602285 Human
- SwissProt: Q9ULZ9 Human
- Unigene: 709245 Human
Anti-MMP17 antibody images:
Western blot - MMP17 antibody (ab39708)
All lanes : Anti-MMP17 antibody (ab39708) at 1/1000 dilution
Lane 1 : SW620 Cell Lysate
Lane 2 : SW620 Cell Lysate
Lysates/proteins at 20 µl per lane.
Predicted band size : 67 kDa
Protocols
References for Anti-MMP17 antibody (ab39708)
ab39708 has not yet been referenced specifically in any publications.
Publishing research using ab39708? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"