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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP19 antibody - Carboxyterminal end
See all MMP19 products (10) ...
Description
Rabbit polyclonal to MMP19 - Carboxyterminal end
Specificity
ab39029 recognises the Carboxyterminal end of MMP19. It does not recognise the smallest active form of MMP19 as the hemopexin domain is cleaved from it. This antibody will bind to the reduced and non reduced form of MMP19, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.)
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to the carboxyterminal end of human MMP19.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 57 kDa. When used against the reduced protein, ab39029 identifies bands at 58 Kd, 45 Kd, and a series of further cleaved active forms. Note: the conditioned media may need to be concentrated for Western blot. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Endopeptidase that degrades various components of the extracellular matrix, such as aggrecan and cartilage oligomeric matrix protein (comp), during development, haemostasis and pathological conditions (arthritic disease). May also play a role in neovascularization or angiogenesis. Hydrolyzes collagen type IV, laminin, nidogen, nascin-C isoform, fibronectin, and type I gelatin.
Tissue specificity
Expressed in mammary gland, placenta, lung, pancreas, ovary, small intestine, spleen, thymus, prostate, testis colon, heart and blood vessel walls. Not detected in brain and peripheral blood leukocytes. Also expressed in the synovial fluid of normal and rheumatoid patients.
Involvement in disease
May play a role in pathological processes participating in rheumatoid arthritis (RA)-associated joint tissue destruction. Autoantigen anti-MMP19 are frequent in RA patients.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
Activated by autolytic cleavage after Lys-97.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionQ99542
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrix metalloproteinase 18 antibody
- Matrix metalloproteinase 19 antibody
- Matrix metalloproteinase RASI antibody
see all
Database links
- Entrez Gene: 4327 Human
- Omim: 601807 Human
- SwissProt: C0M4B0 Human
- SwissProt: Q99542 Human
- Unigene: 154057 Human
- Unigene: 591033 Human
Protocols
References for Anti-MMP19 antibody - Carboxyterminal end (ab39029)
ab39029 has not yet been referenced specifically in any publications.
Publishing research using ab39029? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"