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Overview

  • Product nameMMP2 Human ELISA kitSee all MMP2 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 3.5 ng/ml
  • Range
    3.3 ng/ml - 800 ng/ml
  • Recovery

    81 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 77.25 69% - 92%
    Serum 81.27 70% - 93%
    Plasma 84.53 72% - 95%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam's MMP2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP2 in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended as anticoagulants for use in this assay due to their chelating properties) and cell culture supernates.

     

    This assay employs an antibody specific for human MMP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MMP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.

    • Target information above from: UniProt accession P08253 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        72 kDa gelatinase72 kDa type IV collagenaseCLG 4
        CLG 4A CLG4CLG4ACollagenase type IV AEC 3.4.24.24Gelatinase AGelatinase alpha Gelatinase neutrophil Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)Matrix metalloproteinase 2Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)Matrix metalloproteinase IIMatrix metalloproteinase-2MMP 2MMP IIMMP-2MMP2MMP2_HUMANMONANeutrophil gelatinasePEXTBE 1TBE-1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100606 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      Protocols

      References for MMP2 Human ELISA kit (ab100606)

      ab100606 has not yet been referenced specifically in any publications.

      Publishing research using ab100606 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100606 Submit a Question for ab100606

      Displaying 1 - 10 of 10 results for Abreviews and Q&A

      10
      Votes: Highest First

      MMP2 Human ELISA kit works great

       Excellent Excellent 5/5 (Ease of Use)
      Abreviews
      This kit was very easy to use. I followed the product insert exactly and it worked very well. The results were also reproducible. I would use abcam again for my ELISA or western blotting needs.
      Username

      Verified customer

      Submitted Jun 18 2013

      Dear Technical Team,



      Hope this e-mail finds you well!



      Kindly confirm if the below kits can be used in human Cerebrospinal fluid(CSF) as enquired by the customer:



      · MMP2 Human ELIS...

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      Thank you for your inquiry.

      I can confirm that these two kits have not been experimentally tested with human cerebrospinal fluid and are therefore not guaranteed to work with this kind of sample.

      But we are confident that ab100606 and ...

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      Dear Tech support

      Is any of the following kits specific for the C-terminal of MMP2?
      Could you please provide further information regarding the kit's specificity?

      Thank you for your kind help.
      Regards,

      Thank you for contacting us.

      I now have further information to share with you in regards to the MMP2 ELISA kits, ab100606 and ab100730. The antibodies used within these kits have used the following immunogens:

      ab100606 capture antibody...

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      Please check the below and reply me.
      Our customer would like to order as soon as possible.

      Best regards,


      Please note this product is unpublished so when you decide to order you need to contact our customer service team for them to put this item in your order.

      I hope this info will help. Please let me know if you experience any difficulties.

      Thank you for your quick reply and offer to send us a replacement kit for MMP2. As far as we can judge from the standards looking ok the other kits were not affected but it is difficult to predict whether there was any effect of the buffer as this is the ...

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      Thank you very much for your kind reply.

      I am currently having discussions with developer of this kit regarding the new kit we agreed, sending as a free of charge replacement. This is an extra step for avoiding any further inconvenience due to w...

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      Our customer purchased 6 different kits and those HRP-streptavidin concentrations are vary.
      Let me know the concentration of this new product and if this is available from now.
      Our customer concerns about the concentration because she has to cha...

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      This product is available but not published on our website. You cannot purchase this using our normal ordering system however I can add this product to your next shipment. Could you confirm how many vials customer would need..6?.
      In my last I made an...

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      Our customer is looking forward to your reply.
      Please check this.
      Best regards

      Thank you for contacting us. I apologize for the delay.

      We are ready to publish this product. The product would be available at US dollar 120 per 200ul. The customer would need 6 vials of product so it will cost them $720 excluding shipping char...

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      Dear technical team,

      Our customer purchased
      ab100586(lot.GR15494),
      ab100662(lot.GR41054),
      ab100606(lot.37486),
      ab100610(lot.GR39199),
      ab100653(lot.GR37277) and
      ab100651(lot.GR37276)
      last year.

      She s...

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      Thank you for your email. I am sorry for the delay while I was in contact with lab.

      The volume of HRP-Streptavidin is very small and therefore would be difficult to see with the naked eye. The HRP-streptavidin volume sent was 8ul and is very con...

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      I am interested in purchasing the following ELISAs:
      Ab100606 MMP-2 ELISA £345
      Ab100610 MMP-9 ELISA £345
      Ab100652 TIMP-1 ELISA £345
      Ab100653 TIMP-2 ELISA £345
      Could you let me know if you could provide me wi...

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      Thank you very much for contacting. I apologize for the delay as we were conducting some tests.

      We have also seen gloopy things in the Assay buffer which first seen as like fungal growth however when we warmed the buffer at room temperature whic...

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      I don't know if you are the correct person to contact, if not please could you forward my email to the relevant department.
      I purchased the four ELISA kits listed below on the 23rd March and received them just before Easter (PO 68241150). They have b...

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      Thank you for contacting us. I apologize for the inconvenience caused.

      I have started working on your inquiry and have asked our lab to provide details? I will get back to you with more updates soon.

      Many thanks for having patience! <...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"