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What is the sequence of this peptide, the immunogen for ab38919, and how is it purified? |
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ANSWER: |
We are not able to obtain the exact sequence of the immunogen as it is proprietary, but we know that it falls in the middle of the propeptide domain of the human sequence of MMP-2, starting in the forties and ending in the seventies, that is, somewhere within this sequence: VAPKTDKELAVQYLNTFYGCPKESCNLFVLKDTLKKMQKF The peptide is HPLC-purified. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-MMP2 antibody - Propeptide domain (ab38919) at 1/1000 dilution
Lane 1 : Human MMP2
Lane 2 : Cell Media from human dermal fibroblasts (neonatal) treated with IL1 beta
Predicted band size : 74 kDa
Observed band size : 72 kDa (why is the actual band size different from the predicted?)
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