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Our Abpromise guarantee covers the use of ab37150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).|
|IHC-Fr||Use a concentration of 2 - 4 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20181831|
ab37150 staining MMP2 in Rat fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed and then blocked using 1% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) (ab150077) used at a 1/1000 dilution.Cells were stimulated with PDBu.