Overview

  • Product nameAnti-MMP2 antibody
    See all MMP2 primary antibodies
  • Description
    Rabbit polyclonal to MMP2
  • SpecificityThis antibody is specific for pro and activated forms of MMP2. It does not react with other MMPs tested.
  • Tested applicationsWB, IHC-Fr, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Synthetic peptide corresponding to Human MMP2 aa 475-490.
    Sequence:

    MGPLLVATFWPELPEK


    Database link: P08253

  • Positive control
    • Western blot: injured rat nerve lysate, IHC-FFPE: human neurofibroma section. No antigen retrieval is necessary. IHC-Frozen: injured rat nerve tissue section

Properties

Applications

Our Abpromise guarantee covers the use of ab37150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-Fr Use a concentration of 2 - 4 µg/ml.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20181831
Flow Cyt Use at an assay dependent concentration.

Target

  • FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 72 kDa gelatinase antibody
    • 72kD type IV collagenase antibody
    • CLG 4 antibody
    • CLG 4A antibody
    • CLG 4A antibody
    • CLG4 antibody
    • CLG4A antibody
    • CLG4A antibody
    • Collagenase Type 4 alpha antibody
    • Collagenase Type 4 alpha antibody
    • Collagenase type IV A antibody
    • Gelatinase A antibody
    • Gelatinase alpha antibody
    • Gelatinase alpha antibody
    • Gelatinase neutrophil antibody
    • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody
    • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody
    • Matrix Metalloproteinase 2 antibody
    • Matrix Metalloproteinase 2 antibody
    • Matrix metalloproteinase II antibody
    • Matrix metalloproteinase-2 antibody
    • MMP 2 antibody
    • MMP 2 antibody
    • MMP II antibody
    • MMP-2 antibody
    • MMP2 antibody
    • MMP2_HUMAN antibody
    • MONA antibody
    • Neutrophil gelatinase antibody
    • PEX antibody
    • TBE 1 antibody
    • TBE-1 antibody
    see all

Anti-MMP2 antibody images

  • All lanes : Anti-MMP2 antibody (ab37150) at 0.5 µg/ml

    Lane 1 : recombinant MMP2
    Lane 2 : injured rat nerve lysate

    developed using the ECL technique

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 66 kDa (possible isoform).
  • ab37150 staining MMP2 in Rat fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed and then blocked using 1% BSA for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) (ab150077) used at a 1/1000 dilution.Cells were stimulated with PDBu.

    See Abreview

  • Detection of MMP2 in a paraffin-embedded human neurofibroma section stained with ab37150 at 2µg/ml.
    No antigen retrieval was carried out.
    Detection of MMP2 in a paraffin-embedded human neurofibroma section stained with ab37150 at 2µg/ml.
    No antigen retrieval was carried out.
  • Detection of MMP2 in a frozen injured rat nerve tissue section stained using ab37150 at 2µg/ml.
    No antigen retrieval was carried out.

References for Anti-MMP2 antibody (ab37150)

This product has been referenced in:
  • Moon HJ  et al. Effects of secreted factors in culture medium of annulus fibrosus cells on microvascular endothelial cells: elucidating the possible pathomechanisms of matrix degradation and nerve in-growth in disc degeneration. Osteoarthritis Cartilage 22:344-54 (2014). WB ; Human . Read more (PubMed: 24361793) »
  • Bauters D  et al. Gelatinase B (MMP-9) gene silencing does not affect murine preadipocyte differentiation. Adipocyte 3:50-3 (2014). Read more (PubMed: 24575369) »

See all 23 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HepG2)
Specification HepG2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Nov 10 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 37°C
Antigen retrieval step Enzymatic
Sample Mouse Tissue sections (BRAIN)
Specification BRAIN
Permeabilization No
Fixative Formaldehyde
Username

Ms. sehee oh

Verified customer

Submitted Nov 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Specification Mouse Embryonic Fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Aug 27 2013

Thank you for contacting us.

I understand that you are requesting for a safety datasheet for ab37150. The link to the requested document, available from the online product datasheet (http://www.abcam.com/ab37150), is http://www.abcam.com/ps/Pr...

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As requested, I have attached the datasheet for ab7032 as well as our Mouse on Mouse staining protocol. Abcam also carries a Mouse on Mouse staining kit which contains all of the reagents need to perform mouse ...

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The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality....

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You should not have any issues using ab39304 with ab37150 as a positive control in western blot. Both are covered under our Abpromise for six months and are guaranteed to work in western blot.
If there is anythi...

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Our products are guaranteed for a period of six months after delivery if stored correctly. Products that are kept at -20C are stable for 5 years if stored under constant temperature.


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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (ovary)
Loading amount 25 µg
Specification ovary
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Mrs. Henda Nabli

Verified customer

Submitted Jan 20 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"