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Overview

  • Product nameMMP2 protein (Active)See all MMP2 proteins and peptides ...
  • Protein descriptionRecombinant full length Human MMP2 containing the entire catalytic N terminal domain and the C terminal domain, 552 amino acids, 62 kDa (SwissProt P08253).
  • Expression hostE. coli

    • Properties

  • Purity> 90 % by SDS-PAGE
  • Biological activityBiological Activity : MMP2 activity was measured by its ability to cleave a chromogenic peptide MMP2 substrate at room temperature. At an MMP2 concentration of 2.5 µg/ml, 50% cleavage was achieved at an incubation time of approximately 25 minutes.
  • FormLyophilised:Reconstitute in water to a concentration of 0.1 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Concentration information loading...
  • Additional notesEndotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Sequence notesMYNFFPRKPK WDKNQITYRI IGYTPDLDPE TVDDAFARAF QVWSDVTPLR FSRIHDGEAD IMINFGRWEH GDGYPFDGKD GLLAHAFAPG TGVGGDSHFD DDELWTLGEG QVVRVKYGNA DGEYCKFPFL FNGKEYNSCT DTGRSDGFLW CSTTYNFEKD GKYGFCPHEA LFTMGGNAEG QPCKFPFRFQ GTSYDSCTTE GRTDGYRWCG TTEDYDRDKK YGFCPETAMS TVGGNSEGAP CVFPFTFLGN KYESCTSAGR SDGKMWCATT ANYDDDRKWG FCPDQGYSLF LVAAHEFGHA MGLEHSQDPG ALMAPIYTYT KNFRLSQDDI KGIQELYGAS PDIDLGTGPT PTLGPVTPEI CKQDIVFDGI AQIRGEIFFF KDRFIWRTVT PRDKPMGPLL VATFWPELPE KIDAVYEAPQ EEKAVFFAGN EYWIYSASTL ERGYPKPLTS LGLPPDVQRV DAAFNWSKNK KTYIFAGDKF WRYNEVKKKM DPGFPKLIAD AWNAIPDNLD AVVDLQGGGH SYFFKGAYYL KLENQSLKSV KFGSIKSDWL GC
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab81550 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
  • Application notesThis peptide can be used with studies using ab2462,ab37150,ab79781,ab83173 and ab110186.

    • Protein info

    • Alternative names
        72 kDa gelatinase72kD type IV collagenaseCLG 4
        CLG 4ACLG4CLG4ACollagenase Type 4 alphaCollagenase type IV AGelatinase AGelatinase alphaGelatinase neutrophilMatrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenaseMatrix Metalloproteinase 2Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)Matrix metalloproteinase IIMatrix metalloproteinase-2MMP 2MMP IIMMP-2MMP2MMP2_HUMANMONANeutrophil gelatinasePEXTBE 1TBE-1
      see all
  • FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.

    • Target information above from: UniProt accession P08253 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for MMP2 protein (Active) (ab81550)

    ab81550 has not yet been referenced specifically in any publications.

    Publishing research using ab81550 ? Please let us know so that we can cite the reference in this datasheet

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    I am thinking of purchasing MMP2 protein (Active) is it possible to be told the constituents of the buffer it is supplied in?

    The protein is in a buffer which contains 5mM sodium phosphate pH 7.0 and 0.1mM calcium chloride.

    I am planning to use the MMP-2 to test the activity of my designed substrate. So, I am wondering if this active MMP-2 has been already activated by AMPA? Can I use directly after reciving it or do I need to activate it before use? How long can I store it ...

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    Thank you for your enquiry. I can confirm that ab81550 MMP2 protein (Active) is the fully active protein and no activation is required. The product only needs to be reconstituted and is ready to use. The reconstituted product can be diluted further ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"