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MMP2 protein (Human) (ab39302)

Abreviews (1)Q&A (4)

Overview

  • Product nameMMP2 protein (Human)See all MMP2 proteins and peptides ...
  • Protein descriptionFull length Human MMP2 protein (purified from human fibroblast cell culture media).
  • Expression hostNative

    • Properties

  • Purification notesPurified from human fibroblasts, free of its endogenous inhibitor, TIMP-2, and other matrix metalloproteinases. The enzyme is supplied as a mixture of the zymogen and processed enzyme.
  • Biological activityDegrades denatured Collagen (gelatin), and a range of extracellular matrix components in-vivo.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, PBS, 250mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab39302 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution. Predicted molecular weight: 74 kDa.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        72 kDa gelatinase72kD type IV collagenaseCLG 4
        CLG 4ACLG 4ACLG4CLG4ACLG4ACollagenase Type 4 alphaCollagenase Type 4 alphaCollagenase type IV AGelatinase AGelatinase alphaGelatinase alphaGelatinase neutrophilMatrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenaseMatrix Metalloproteinase 2Matrix Metalloproteinase 2Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)Matrix metalloproteinase IIMatrix metalloproteinase-2MMP 2MMP 2MMP IIMMP-2MMP2MMP2_HUMANMONANeutrophil gelatinasePEXTBE 1TBE-1
      see all
  • FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
    -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
    PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
  • Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
  • Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 3 fibronectin type-II domains.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications    Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
    The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
  • Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.

    • Target information above from: UniProt accession P08253 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for MMP2 protein (Human) (ab39302)

    ab39302 has not yet been referenced specifically in any publications.

    Publishing research using ab39302 ? Please let us know so that we can cite the reference in this datasheet

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    Western blot Abreview for MMP2 protein (Human)

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    Abreviews
    Application Western blot

    Sample: Human Purified protein (human MMP2 protein from abcam)
    Loading amount: 0.32 µg
    Specification: human MMP2 protein from abcam
    Gel Running Conditions: Reduced Denaturing (12%)
    Blocking step: Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

    Other product details
    Incubation time: 18 hour(s) and 0 minute(s)

    Primary antibody (in addition to 'MMP2 protein (Human)')
    Primary antibody: Abcam primary antibody: Anti-MMP2 antibody (ab37150)
    Dilution: 1/500

    Secondary antibody
    Name: Non-Abcam antibody was used: biotinylated anti-rabbit
    Host species: Goat
    Clonality: Polyclonal
    Conjugation: Biotin
    Dilution: 1/200

    Detection
    Detection method: colorimetric
    Exposure: 5 minute(s) and 0 second(s)
    Bands: Specific: 74 kDa
    Positive control: ovarian lysates
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    Verified customer

    Submitted Nov 07 2012

    I am looking for recombinant proteins for MMP-2 and MMP-9 for gelatinzymography.

    Do you have any and if yes how much are they?

    Thank you for contacting us.

    We do have several MMP proteins in our catalog.

    We have for MMP2:

    ab39302 (human):Full length Human MMP2 protein (purified from human fibroblast cell culture media). It has not yet been tested in...

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    Dear I’m sorry to bother you, Thanks for your kindly reply; I attached the experiment step in this letter, this customer would likes to ask for your help not only ab39302 (MMP2 protein (Human)) but also ab39308 (MMP9 protein (Human)), could you please hel...

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    Sorry about the delay in the response, I have been consulting with the lab to see what the problem might be. It seems that the antibodies used in the detection could be quite crucial. Could you tell me exatcly which antibodies the customer used with the t...

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    Dear technical support: This customer has purchased ab39302 (MMP2 protein (Human)) and he raised an enquire about his wb data which has wrong size , Moreover according to the datasheet, this antibody’s predicted size should be 76kDa, but the wb data...

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    Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am attachin...

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    Dear technical support: Our lab is highly interested in ab39302 (MMP2 protein (Human)) and ab39308 (MMP9 protein (Human)), we wants to know whether these proteins have any test for activity assay record?  If yes, could you please send the report for th...

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    Thank you for contacting us. We do not have any activity assay data related to these products. However I can confirm that these products are active and can be used as enzymes. Ab39302 and ab39308; actively degrades denatured Collagen (gelat...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"