MMP2 protein (Rat) (ab39304)

Did not see a band in WB with this protein. Antibody works with a mouse recombinant protein though.

ANSWER:

Thank you for your call today and for letting us know about the trouble with this protein.

I had requested that a replacement vial be sent to you, but your colleague kindly called to let us know that we have previously credited your account for the original purchase so I am unable to send a replacement for ab39304. If you would like to try a vial from a new lot, you may purchase itthrough your original means, and if it also does not work in Western blot or zymography we would of course be happy to credit you for it.

I apologize for my error on the phone earlier, but please let me know if you have any quesitons or if there is anything else that we can do for you. Have a good weekend.

Thanks for your email. I will accept thecredit note as compensation.

Thanks for looking into this for me.

ANSWER:

Thank you for your reply and understanding.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you.

Credit note ID: #####

The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Hi,

I purchased a MMP-2 protein (ab39304).
After using it for zymography I found I have 2 bands when I only expected one. I believe the upper band maybe MMP-9 and the lower MMP-2. Has anyone else found this for this product? Also, would I be able to get some extra information regarding how this product was made, how MMP-2 was purified etc.

Thanks,

ANSWER:

Thank you for your enquiry and for your patience. I am sorry to hear you have some concerns regarding this product.

I can confirm that this particular product is sourced externally. I have been in contact with them in order to obtain this information for you, and regrettably have not yet recieved a reply.

Particularly as you have had difficulties with this product, It is disappointing we have not yet recieved a response for you in this case. We aim to provide as much information as possible to customers within 24 hours of receiving an enquiry. For those products we source externally, there can sometimes be a delay. However, most sources are excellent and will provide us with requested extra information very quickly.

Regrettably, the originator for this product, although a trusted supplier with regards to product quality and delivery, can sometimes take longer to get back to us regarding technical enquiries. We have previously raised this issue with them. We also constantly monitor our supplier performance on a monthly basis with regards to both quality of the products and the support they provide. Actions are taken with our sources when necessary and feedback has been forwarded to the relevant people in this case.

I am sincerely sorry there has been such a delay in this case. In order to manage your expectations at this time, I can suggest it may be that they have not responded yet because they do not have this information for this particular product.

Therefore. I would like to offer you a free of charge replacement orcredit note in compensation.

If you require anything further, please do not hesitate to let me know. I look forward to hearing from you with details of how you would like ot proceed.

I sent this email a week ago and I was told that this was being looked into. I was wondering if you have determined anything yet. Thanks

Hi,

I purchased a MMP-2 protein (ab39304).

After using it for zymography I found I have 2 bands when I only expected one. I believe the upper band maybe MMP-9 and the lower MMP-2. Has anyone else found this for this product? Also, would I be able to get some extra information regarding how this product was made, how MMP-2 was purified etc.

Thanks,

ANSWER:

Thank you for your message and for your patience.

I sincerely apologise for the delay. I have contacted our source to request the information regarding the purification of the product, but they have regrettably not yet got back to me. I will contact them again today and let you know as soon as I have further information.
Regarding the zymography, this protein has not been used in this application before. All tested and guaranteed applications are listed on the datasheets. In this case, I am sorry we are regrettably not able to provide our guarnateefor zymography or provide any other information regarding zymography results.

I will get back to you as soon as I have more details. If you have any questions in the meantime, please do not hesitate to contact me.

I am looking for recombinant proteins for MMP-2 and MMP-9 for gelatinzymography.

Do you have any and if yes how much are they?

ANSWER:

Thank you for contacting us.

We do have several MMP proteins in our catalog.

We have for MMP2:

ab39302 (human):Full length Human MMP2 protein (purified from human fibroblast cell culture media). It has not yet been tested in zymopgraphy. (10 ug for $274.00)
http://www.abcam.com/MMP2-protein-Human-ab39302.html

ab39304 (rat): purified from rat lung epithelial cell culture media. The enzyme is supplied as a mixture of the zymogen and processed enzyme. This protein was purified from rat lung epithelials, and is free of its endogenous inhibitor, TIMP2, and other matrix metalloproteinases.
It has been tested in zymopraphy. (10 ug for $274.00)
http://www.abcam.com/MMP2-protein-Rat-ab39304.html

We have for MMP9, but none of these proteins were tested in zymography:

ab39308 (human): Purified from cell culture media of stimulated human fibroblasts, this protein is free of its endogenous inhibitor, TIMP1, and other matrix metalloproteinases. (10 ug for $274.00)
http://www.abcam.com/MMP9-protein-Human-ab39308.html

ab39309 (mouse): Metalloproteinase purified from cell culture media of BHK cells transfected with Murine 105 kD Gelatinase (murine homologue of Human MMP9). Purified from cell culture media of BHK cells, free of its endogenous inhibitor, TIMP1, and other matrix metalloproteinases.
(10 ug for $274.00)
http://www.abcam.com/MMP9-protein-Mouse-ab39309.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.