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Immunocytochemistry/ Immunofluorescence-MMP20 antibody - Aminoterminal end(ab39038)
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Product Name
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MMP20 antibody - Aminoterminal end
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See all MMP20 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MMP20 - Aminoterminal end
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Immunogen
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Synthetic peptide based on the aminoterminal end of active human MMP20. (Peptide available as ab41111.)
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is specific for MMP20, but does not cross react with the other MMP family members MMP-1, MMP-2, MMP-3, MMP-9, etc.).
We have a range of domain specific antibodies for this target. For a full list please see all MMP20 antibodies
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Tested applications
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ICC/IF, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Detects a band of approximately 58kDa and 45kDa and a series of further cleaved active forms (predicted molecular weight: 58 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted; extracellular space; extracellular matrix.
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
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Relevance
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Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP20 degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). It may play a central role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes that localizes to chromosome 11q22.3.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP20 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MMP20 antibody - Aminoterminal end images, references, products related to ab39038 and other tools.
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MMP20 antibody - Aminoterminal end images:
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 Immunocytochemistry/ Immunofluorescence-MMP20 antibody - Aminoterminal end(ab39038)
ICC/IF image of ab39038 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39038, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to MMP20
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MMP20 antibody - Aminoterminal end - more information
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MMP20 antibody - Aminoterminal end - related products:
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