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- Secondary antibodies
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Product name
Anti-MMP20 antibody
See all MMP20 products (6) ...
Description
Rabbit polyclonal to MMP20
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Pig
Immunogen
Synthetic peptide corresponding to a region within the internal amino acids 396-445 (AAVYLREPQK TLFFVGDEYY SYDERKRKME KDYPKNTEEE FSGVNGQIDA) of Human MMP20 (NP_004762).
Positive control
HepG2 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
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Western blot - MMP20 antibody (ab84737)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 54 kDa).Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Target
Function
Degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). May play a central role in tooth enamel formation.
Tissue specificity
Expressed specifically in the enamel organ.
Involvement in disease
Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2) [MIM:612529]. AI2A2 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational
modifications
Autoactivates at least at the 107-Asn-
-Tyr-108 site.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionO60882
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI2A2 antibody
- Enamel metalloproteinase antibody
- Enamelysin antibody
see all
Database links
- Entrez Gene: 9313 Human
- Entrez Gene: 30800 Mouse
- Entrez Gene: 300341 Rat
- Omim: 604629 Human
- SwissProt: O60882 Human
- SwissProt: P57748 Mouse
- Unigene: 591946 Human
- Unigene: 390121 Mouse
Anti-MMP20 antibody images:
Western blot - MMP20 antibody (ab84737)
Anti-MMP20 antibody (ab84737) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 54 kDa
Observed band size : 43 kDa (why is the actual band size different from the predicted?)
12% gel
References for Anti-MMP20 antibody (ab84737)
ab84737 has not yet been referenced specifically in any publications.
Publishing research using ab84737? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"