MMP21 antibody [EP1277Y] (ab52817)

Product Name 

MMP21 antibody [EP1277Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1277Y] to MMP21

Immunogen 

Synthetic peptide that corresponds to residues from human MMP21.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab52817 recognizes 2 isoforms of MMP21.

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/40.
ICC/IF: 1/50 - 1/100.
IHC-P: 1/100 - 1/250. Antigen retrieval recommended.
IP: 1/30.
WB: 1/200,000. This antibody recognizes 2 isoforms of MMP21, 58kDa and 45kDa bands are detected. (predicted molecular weight: 65 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human kidney lysate and hepatocellular carcinoma tissue.

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP

Relevance 

This protein is a member of the matrix metalloproteinase (MMP) family. MMP21 was first described as an MMP in Xenopus (XMMP), expressed in early embryo development. Later, species orthologs were reported in the Newt Cynops, and in rat, mouse and human. A literature alias was initiated by the deposit of sequences for MMP21/22, which were later renamed MMP23, the cysteine Array MMP (CAMMP). The mouse sequence seems also to be expressed early in embryo development, especially in the brain, and the human sequence has been reported in tumors. MMP21 contains the canonical MMP elements of cysteine switch (PRCGVPD) and HExGHxxxxxH catalytic zinc site, without a transmembrane sequence. The Xenopus and Cynops sequences contain a potential furin like cleavage site (RR/KKR), shortly after the cysteine switch, but the rat, mouse and human furin sites may not be functional. The hinge region is much shorter in MMP21 than in other MMPs, and the carboxyterminal half has some homology to vitronectin. Little is known about MMP21 function, expression or distribution. The relative low homology with other MMPs suggests a different distribution, and the regulatory elements known suggest quite different promotion than “classical” MMPs. The Xenopus and Cynops sequences have two inserts relative to the human and murine sequences, one in the propeptide domain, and one right after the cysteine switch. Interestingly, the pI for the human and murine proteins is quite basic, 9.334, 9.801 and 9.294 respectively for human, rat and mouse, while the Xenopus and Cynops predicted pI are 7.674 and 7.416. The basic pI and vitronectin homology suggest ECM localization. The 569 amino acid human zymogen has a predicted mass of 65.01 kD, the mouse and rat sequences at 65.45 and 61.07 kD, and the Xenopus and Cynops both about 70 kD, without posttranslational modifications.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1277Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at -20°C. Stable for 12 months at -20°C

Notes 

Produced under U.S. Patent No. 5,675,063.

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