MMP21 antibody (ab11636)

Product Name 

MMP21 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP21

Immunogen 

Synthetic peptide (Human) C-term.

Reacts with 
(species key)

Hu, Ms, Rat, Xl

Specificity 

The antibody specifically binds to MMP-21 and does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.).

Tested applications 
(see key)

WB

Abreviews 

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Application notes 
(see key)

Recommended dilutions
WB: Use at an assay dependent dilution. A minimum working antibody dilution of 1/1000 is determined by blotting a tissue cell lysate with an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. A starting dilution of 1/5000 of ab11636 is recommended for chemiluminescent substrates. By blotting against the reduced protein, the antibody identifies bands at 70 kDa and 66 kDa. This is larger than the predicted 65 kDa and possibly represents alternative splicing, posttranslational modifications, aggregation, or other
phenomenon.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP

Relevance 

This protein is a member of the matrix metalloproteinase (MMP) family. MMP21 was first described as an MMP in Xenopus (XMMP), expressed in early embryo development. Later, species orthologs were reported in the Newt Cynops, and in rat, mouse and human. A literature alias was initiated by the deposit of sequences for MMP21/22, which were later renamed MMP23, the cysteine Array MMP (CAMMP). The mouse sequence seems also to be expressed early in embryo development, especially in the brain, and the human sequence has been reported in tumors. MMP21 contains the canonical MMP elements of cysteine switch (PRCGVPD) and HExGHxxxxxH catalytic zinc site, without a transmembrane sequence. The Xenopus and Cynops sequences contain a potential furin like cleavage site (RR/KKR), shortly after the cysteine switch, but the rat, mouse and human furin sites may not be functional. The hinge region is much shorter in MMP21 than in other MMPs, and the carboxyterminal half has some homology to vitronectin. Little is known about MMP21 function, expression or distribution. The relative low homology with other MMPs suggests a different distribution, and the regulatory elements known suggest quite different promotion than “classical” MMPs. The Xenopus and Cynops sequences have two inserts relative to the human and murine sequences, one in the propeptide domain, and one right after the cysteine switch. Interestingly, the pI for the human and murine proteins is quite basic, 9.334, 9.801 and 9.294 respectively for human, rat and mouse, while the Xenopus and Cynops predicted pI are 7.674 and 7.416. The basic pI and vitronectin homology suggest ECM localization. The 569 amino acid human zymogen has a predicted mass of 65.01 kD, the mouse and rat sequences at 65.45 and 61.07 kD, and the Xenopus and Cynops both about 70 kD, without posttranslational modifications.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

Affinity isolated antigen specific antibody is obtained from rabbit anti-MMP21 antiserum by immuno-specific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to the peptide.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Notes 

Do not store below -22 °C.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

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