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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Overview
Product name
Anti-MMP22 antibody - Propeptide domain
See all MMP22 products (5) ...
Description
Rabbit polyclonal to MMP22 - Propeptide domain
Specificity
This antibody is specific for MMP22, and does not react with other family members (MMP1, MMP2, MMP3, MMP9, etc.).
Tested applications
Cross reactivity
Reacts with
Chicken
Immunogen
Synthetic peptide based on the propeptide domain of the chicken MMP22 sequence.
(Peptide available as ab41118.)
Positive control
Cell media from human fetal fibroblasts and mouse lung tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
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Western blot - MMP22 antibody (ab39082)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for non human samples. Detects bands of approximately 70kDa and 66kDa when used against the reduced protein. It is unclear if this represents alternative splicing, post translational modifications, aggregation, or some other phenomenon. (Predicted molecular weight: 53 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Protease. May regulate the surface expression of some potassum channels by retaining them in the endoplasmic reticulum.
Tissue specificity
Predominantly expressed in ovary, testis and prostate.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Domain
The toxin-like domain associates with, and blocks several potassium channels in the nanomolar to low micromolar range. The relative affinity is Kv1.6 > Kv1.3 > Kv1.1 = Kv3.2 > Kv1.4.
Post-translational
modifications
N-glycosylated.
Proteolytic cleavage might yield an active form.
Cellular localization
Endoplasmic reticulum membrane. Membrane. A secreted form produced by proteolytic cleavage may also exist.
Target information above from: UniProt accessionO75900
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- soluble form antibody
- Femalysin antibody
- Matrix metallopeptidase 21 antibody
see all
Database links
- Entrez Gene: 419407 Chicken
Anti-MMP22 antibody - Propeptide domain images:
Western blot - MMP22 antibody (ab39082)
All lanes : Anti-MMP22 antibody - Propeptide domain (ab39082) at 1/1000 dilution
Lane 1 : Cell media from human fetal fibroblasts.
Lane 2 : Mouse lung tissue.
Predicted band size : 53 kDa
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
It is unclear if the actual bands seen are from alternative splicing, posttranslational modifications, aggregation, or some other phenomenon.
Protocols
References for Anti-MMP22 antibody - Propeptide domain (ab39082)
ab39082 has not yet been referenced specifically in any publications.
Publishing research using ab39082? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"