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Product name
Anti-MMP24 antibody - Aminoterminal end active enzyme
See all MMP24 products (6) ...
Description
Rabbit polyclonal to MMP24 - Aminoterminal end active enzyme
Specificity
Ab39695 recognizes the active and pro- forms of MMP24. The antibody binds to MT5-MMP, but does not cross react with the other MMP family members (MMP-1, MMP-2, MMP-3, MMP-9, etc.).
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic peptide based on the sequence of the aminoterminal end of furin-cleaved active Human MMP24.
(Peptide available as ab431 16.)
Positive control
MMP-24 has been reported to be elevated in several tumor cell lines, and is constituitively produced by some normal cell lines. MMP-24 is also found in brain tissue, along with other MT-MMP enzymes.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
WB: 1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
Target
Relevance
Matrix Metalloproteinase 24 (MMP24, MT5MMP) has been described as a proteoglycanase, which is critical in the turnover of ECM (extracellular matrix) components in the brain. MMP24 contains a candidate signal sequence, a propeptide region, a potential furin recognition motif, a zinc-binding catalytic domain, a hemopexin-like domain, a 24-residue hydrophobic region, which acts as a potential transmembrane domain, and a short cytosolic domain. Unlike other MTMMPs, MMP24 (MT5MMP) sheds from the cell surface as soluble proteinases, allowing its versatility as both a cell bound and soluble proteinase in ECM remodeling processes. MMP24 is elevated in several tumor cell lines, and is also produced by some normal cell lines. It is predominantly expressed in the brain, indicating a role in nervous system development. MMP24 is also highly expressed during embryonic development. The mouse MMP24 gene maps to chromosome 2.
Cellular localization
Cell membrane; single-pass type I membrane protein. Processed form: Secreted protein. Also shed from cell surface as soluble proteinase, by a proteolytic cleavage.
Alternative names
- Matrix metallopeptidase 24 (membrane inserted) antibody
- Matrix metallopeptidase 24 antibody
- membrane-type 5 matrix metalloproteinase antibody
see all
Database links
- Entrez Gene: 10893 Human
- Entrez Gene: 85513 Rat
- Omim: 604871 Human
- SwissProt: Q9Y5R2 Human
- SwissProt: Q99PW6 Rat
- Unigene: 212581 Human
- Unigene: 3117 Rat
Anti-MMP24 antibody - Aminoterminal end active enzyme images:
Western blot - MMP24 antibody - Aminoterminal end active enzyme (ab39695)
All lanes : Anti-MMP24 antibody - Aminoterminal end active enzyme (ab39695) at 1/1000 dilution
Lane 1 : SF treated SW 1353 CM
Lane 2 : TPA treated SW 1353 CM
Lane 3 : IL1-beta treated SW 1353 CM
Lane 4 : RA treated SW 1353 CM
Lysates/proteins at 15 µl per lane.
Predicted band size : 65 kDa
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - MMP24 antibody - Aminoterminal end active enzyme (ab39695)
ab39695 staining rat P5 brain by ICC/IF. The cells were paraformaldehyde fixed and blocked with serum before incubation with the antibody for 12 hours at 4°C. An Alexa Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Shekhar Patil
Protocols
References for Anti-MMP24 antibody - Aminoterminal end active enzyme (ab39695)
ab39695 has not yet been referenced specifically in any publications.
Publishing research using ab39695? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"