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Product name
Anti-MMP24 antibody - Cytoplasmic domain
See all MMP24 products (6) ...
Description
Rabbit polyclonal to MMP24 - Cytoplasmic domain
Specificity
Ab11647 specifically binds to MMP24 and does not cross-react with the other MMP family members (MMP1, MMP2, MMP3, MMP9). The cytoplasmic domain of MMP24 is detached when MTMMPs shed from the membrane, thus this antibody should not detect these forms of MMP24.
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to the cytoplasmic domain of mouse MMP24. (Peptide available as ab411 24.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
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Western blot - MMP24 antibody (ab11647)
(enlarge)
Applications
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Our Abpromise guarantee covers the use of ab11647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: A recommended starting concentration for Western blots is 1:2,000 when using colorimetric substrates such as BCIP/NBT and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples The antibody identifies a band at 65 kD (zymogen) and also the activation and breakdown products. Ab11647 also recognizes non-reduced (native) MMP24. Note: Since cell types differ greatly in the quantity of MMP24 produced, the conditioned media may require mitogen stimulation to visualize the bands by Western blotting. Treatment of cells with concanavolin-A or the phorbol ester TPA stimulates production of MMP24 in some cell types, and the enzyme can be recovered in cell lysates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Matrix Metalloproteinase 24 (MMP24, MT5MMP) has been described as a proteoglycanase, which is critical in the turnover of ECM (extracellular matrix) components in the brain. MMP24 contains a candidate signal sequence, a propeptide region, a potential furin recognition motif, a zinc-binding catalytic domain, a hemopexin-like domain, a 24-residue hydrophobic region, which acts as a potential transmembrane domain, and a short cytosolic domain. Unlike other MTMMPs, MMP24 (MT5MMP) sheds from the cell surface as soluble proteinases, allowing its versatility as both a cell bound and soluble proteinase in ECM remodeling processes. MMP24 is elevated in several tumor cell lines, and is also produced by some normal cell lines. It is predominantly expressed in the brain, indicating a role in nervous system development. MMP24 is also highly expressed during embryonic development. The mouse MMP24 gene maps to chromosome 2.
Cellular localization
Cell membrane; single-pass type I membrane protein. Processed form: Secreted protein. Also shed from cell surface as soluble proteinase, by a proteolytic cleavage.
Alternative names
- Matrix metallopeptidase 24 (membrane inserted) antibody
- Matrix metallopeptidase 24 antibody
- membrane-type 5 matrix metalloproteinase antibody
see all
Database links
- Entrez Gene: 10893 Human
- Entrez Gene: 17391 Mouse
- Omim: 604871 Human
- SwissProt: Q9Y5R2 Human
- SwissProt: Q9R052 Mouse
- Unigene: 212581 Human
Anti-MMP24 antibody - Cytoplasmic domain images:
Western blot - MMP24 antibody (ab11647)
Anti-MMP24 antibody - Cytoplasmic domain (ab11647)
Predicted band size : 65 kDa
Observed band size : 65 kDa
Cell Media from human dermal fibroblasts, neonatal (treated with II1alpha).
Protocols
References for Anti-MMP24 antibody - Cytoplasmic domain (ab11647)
ab11647 has not yet been referenced specifically in any publications.
Publishing research using ab11647? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"