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- Proteins and Peptides
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Product name
Anti-MMP24 antibody - Hinge region
See all MMP24 products (6) ...
Description
Rabbit polyclonal to MMP24 - Hinge region
Specificity
This antibody binds to the hinge region of MMP24. This antibody can recognize native or reduced MMP24, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9).
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide of Human MMP24 corresponding to the hinge region.
(Peptide available as ab411 26.)
Positive control
Cell media from embryonal human rhabdomyosarcoma.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
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Western blot - MMP24 antibody - Hinge region (ab39137)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Detects a band of approximately 65 kDa (predicted molecular weight: 73 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Matrix Metalloproteinase 24 (MMP24, MT5MMP) has been described as a proteoglycanase, which is critical in the turnover of ECM (extracellular matrix) components in the brain. MMP24 contains a candidate signal sequence, a propeptide region, a potential furin recognition motif, a zinc-binding catalytic domain, a hemopexin-like domain, a 24-residue hydrophobic region, which acts as a potential transmembrane domain, and a short cytosolic domain. Unlike other MTMMPs, MMP24 (MT5MMP) sheds from the cell surface as soluble proteinases, allowing its versatility as both a cell bound and soluble proteinase in ECM remodeling processes. MMP24 is elevated in several tumor cell lines, and is also produced by some normal cell lines. It is predominantly expressed in the brain, indicating a role in nervous system development. MMP24 is also highly expressed during embryonic development. The mouse MMP24 gene maps to chromosome 2.
Cellular localization
Cell membrane; single-pass type I membrane protein. Processed form: Secreted protein. Also shed from cell surface as soluble proteinase, by a proteolytic cleavage.
Alternative names
- Matrix metallopeptidase 24 (membrane inserted) antibody
- Matrix metallopeptidase 24 antibody
- membrane-type 5 matrix metalloproteinase antibody
see all
Database links
- Entrez Gene: 10893 Human
- Entrez Gene: 17391 Mouse
- Omim: 604871 Human
- SwissProt: Q9Y5R2 Human
- SwissProt: Q9R052 Mouse
- Unigene: 212581 Human
Anti-MMP24 antibody - Hinge region images:
Western blot - MMP24 antibody - Hinge region (ab39137)
Anti-MMP24 antibody - Hinge region (ab39137) at 1/1000 dilution + Cell media from embryonal human rhabdomyosarcoma.
Performed under reducing conditions.
Predicted band size : 73 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-MMP24 antibody - Hinge region (ab39137)
ab39137 has not yet been referenced specifically in any publications.
Publishing research using ab39137? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"