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Anti-MMP28 antibody - Carboxyterminal end (ab39183)

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Overview

  • Product nameAnti-MMP28 antibody - Carboxyterminal endSee all MMP28 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP28 - Carboxyterminal end
  • SpecificityThe antibody binds to MMP-28, but does not cross react with the other MMP family members.
  • Tested applicationsSandwich ELISA, WB more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide of MMP28 based on the carboxyterminal end of the Human sequence.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab41143.)

  • Positive controlMMP-28 has been detected in the following tissues: testis, heart, brain, placenta, lung, prostate, intestine, colon, lung, kidney, brain, skeletal muscle, and several tumor cell lines.

Properties

Applications

Our Abpromise guarantee covers the use of ab39183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA
WB
  • Application notesWB: 1/1000 - 1/5000. Use under non reducing conditions. Detects a bands of approximately 58 kDa and 62 kDa. (Breakdown products seen at 50, 48 and 46 kD with bands at 64 kD and 30 kD are also seen in some cell lines). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionCan degrade casein. Could play a role in tissues homeostasis and repair.
    • Tissue specificityExpressed at high levels in testes and lung. Low levels are detected in kidney, pancreas and skin. Also expressed in fetal lung, brain, skeletal muscle and kidney. Expressed selectively in keratinocytesis. Widely expressed in several carcinomas as well. Is up-regulated in response to injury in the skin.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications      The precursor is cleaved by a furin endopeptidase.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.

      • Target information above from: UniProt accession Q9H239 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        Epilysin antibodyEpilysin antibodyMatrix Metalloproteinase 28 antibody
        Matrix Metalloproteinase 28 antibodyMatrix metalloproteinase-28 antibodyMM 28 antibodyMM28 antibodyMMP 25 antibodyMMP 28 antibodyMMP-28 antibodyMMP25 antibodyMMP28 antibodyMMP28_HUMAN antibody
      see all

    Anti-MMP28 antibody - Carboxyterminal end images

    • Sandwich ELISA - MMP28 antibody - Carboxyterminal end (ab39183)
      Sandwich ELISA - MMP28 antibody - Carboxyterminal end (ab39183)
      Standard curve for MMP28; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal to MMP28 (ab57724) at 5µg/ml and Detector Antibody aRabbit polyclonal to MMP28 - Carboxyterminal end (ab39183) at 0.5µg/ml.
    • Western blot - MMP28 antibody (ab39183)
      Western blot - MMP28 antibody (ab39183)
      All lanes : Anti-MMP28 antibody - Carboxyterminal end (ab39183) at 1/1000 dilution

      Lane 1 : Human keratinocyte cell lysate (no treatment)
      Lane 2 : Mouse hide tissue lysate


      Performed under reducing conditions.

      Predicted band size : 58, 62 kDa
      Observed band size : 58,62 kDa

    References for Anti-MMP28 antibody - Carboxyterminal end (ab39183)

    ab39183 has not yet been referenced specifically in any publications.

    Publishing research using ab39183 ? Please let us know so that we can cite the reference in this datasheet

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