MMP3 Human ELISA Kit (ab100607)
- Product nameMMP3 Human ELISA KitSee all MMP3 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 0.3 ng/ml
- Range0.41 ng/ml - 100 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 94.72 84% - 103% Serum 95.39 83% - 103% Plasma 96.25 85% - 104%
- Species reactivityReacts with: Human
- Product overview
ab100607 MMP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP3 pro and active forms in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended) and cell culture supernatants.
This assay employs an antibody specific for human MMP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion tothe amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent 5x concentrated buffer. ForStandard/Sample (serum/plasma samples/cell culture medium/urine) diluent. 1 x 15ml Detection Antibody MMP-3: biotinylated anti-human MMP-3 2 vials HRP-Streptavidin Concentrate 1400x concentrated HRP-conjugated streptavidin 1 x 200µl MMP 3 Microplate (12 strips x 8 wells) coated with anti-human MMP-3 1 unit MMP 3 Standard Human (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
Contains 4 hemopexin-like domains.
- CHDS6Matrix metalloproteinase 3 Matrix metalloproteinase 3 preproprotein
- Matrix metalloproteinase-3MGC126102MGC126103MGC126104MMP 3MMP-3MMP3MMP3_HUMANProgelatinaseProteoglycanaseSL 1 SL-1SL1STMYSTMY1STR1 Stromelisin 1 Stromelysin 1Stromelysin 1 progelatinase Stromelysin-1Transin 1Transin-1
Our Abpromise guarantee covers the use of ab100607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
MMP3 Human ELISA Kit images
The graph shows the differing levels of MMP3 released by human primary fibroblasts (200µg) exposed to several different active ingredients dissolved in media for several days prior to media collection and analysis.
References for MMP3 Human ELISA Kit (ab100607)
ab100607 has not yet been referenced specifically in any publications.