Overview

  • Product nameMMP3 Human ELISA Kit
    See all MMP3 kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 0.3 ng/ml
  • Range
    0.41 ng/ml - 100 ng/ml
  • Recovery

    95 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.72 84% - 103%
    Serum 95.39 83% - 103%
    Plasma 96.25 85% - 104%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s MMP3 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP3 pro and active forms in serum, plasma, and cell culture supernatants. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended).

    This assay employs an antibody specific for Human MMP3 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • Involvement in diseaseDefects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6) [MIM:614466]. A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. Note=A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names
    • CHDS6
    • matrix metallopeptidase 3 (stromelysin 1, progelatinase)
    • Matrix metalloproteinase 3
    • Matrix metalloproteinase 3 preproprotein
    • Matrix metalloproteinase-3
    • MGC126102
    • MGC126103
    • MGC126104
    • MMP 3
    • MMP-3
    • MMP3
    • MMP3_HUMAN
    • Progelatinase
    • Proteoglycanase
    • SL 1
    • SL-1
    • SL1
    • STMY
    • STMY1
    • STR1
    • Stromelisin 1
    • Stromelysin 1
    • Stromelysin 1 progelatinase
    • Stromelysin-1
    • Transin 1
    • Transin-1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

MMP3 Human ELISA Kit images

  • Representative Standard Curve using ab100607.

Protocols

References for MMP3 Human ELISA Kit (ab100607)

ab100607 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for clarifying this so quickly.
I canceled the order with the free of charge replacement and edited a credit note reference CN ********.
Sorry for my mistake and thank you for being so quick with your reply.

Thank you for kindly confirming these details.
As requested, we arranged a free of charge replacement (2 units of kit ab100607).
Thank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

Thank you for your email.

I am happy to process the replacement. Could you please confirm how many kits (ab100607) your customer ordered originally?

Thank you.

Thank you very much for confirming all these details. This is indeed very useful for us.
It looks like the customer has received a kit with a faulty HRP / HRP conjugate. We will therefore be happy to replace the kit for the customer. Please let us...

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rmmarize the problem. I am sorry to hear that this kit is not providing satisfactory results.
We have not yet received any negative feedback and I am not able yet to tell where the problem might is coming from. I would therefore appreciate if you ...

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Thank you for your interest in the MMP-3 and IL23 kits.

The volume of sample required for each test is 100ul per well of the assay plate.

Regarding the 100% discount, I want to be sure you understand the offer, which is also descr...

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Abreview for MMP3 Human ELISA Kit

Good Good 4/5 (Ease of Use)
Abreviews

Sample: Human Cell (Skin fibroblasts)
Specification: Skin fibroblasts
Amount of Sample: 200 µg
Type of Kit Used: ELISA
Positive control: Included in kit as part of standard curve.
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Incubation times are a little longer than in some other kits, which must be remembered when planning multiple experiments, but the data generated is of a better quality overall.

The graph shows the differing levels of MMP3 released by primary fibroblasts exposed to several different active ingredients dissolved in media for several days prior to media collection and analysis.

(Sorry I cant say what the actives actually are due to business nature of the work).
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Abcam user community

Verified customer

Submitted Feb 09 2012

Thank you for your interest in our kits. I will send the product inserts first thing tomorrow morning, EST. The kits will be compatible with the automated ELISA machine, if the automated set-up approximates the conditions specified in the kit protocols...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"