You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameMMP3 Mouse ELISA kitSee all MMP3 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 10 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 117.4 106% - 130%
    Serum 120.6 101% - 140%
    Plasma 111.6 101% - 137%

  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab100731 MMP3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP3 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse MMP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The StopSolution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • Involvement in diseaseDefects in MMP3 are the cause of susceptibility to coronary heart disease type 6 (CHDS6) [MIM:614466]. A multifactorial disease characterized by an imbalance between myocardial functional requirements and the capacity of the coronary vessels to supply sufficient blood flow. Decreased capacity of the coronary vessels is often associated with thickening and loss of elasticity of the coronary arteries. Note=A polymorphism in the MMP3 promoter region is associated with the risk of coronary heart disease and myocardial infarction, due to lower MMP3 proteolytic activity and higher extracellular matrix deposition in atherosclerotic lesions.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P08254 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CHDS6Matrix metalloproteinase 3 Matrix metalloproteinase 3 preproprotein
        Matrix metalloproteinase-3MGC126102MGC126103MGC126104MMP 3MMP-3MMP3MMP3_HUMANProgelatinaseProteoglycanaseSL 1 SL-1SL1STMYSTMY1STR1 Stromelisin 1 Stromelysin 1Stromelysin 1 progelatinase Stromelysin-1Transin 1Transin-1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100731 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      MMP3 Mouse ELISA kit images

      • ELISA - MMP3 Mouse ELISA kit (ab100731)
        ELISA - MMP3 Mouse ELISA kit (ab100731)

      Protocols

      References for MMP3 Mouse ELISA kit (ab100731)

      ab100731 has not yet been referenced specifically in any publications.

      Publishing research using ab100731 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100731 Submit a Question for ab100731

      Displaying 1 - 1 of 1 results for Abreviews and Q&A

      10
      Votes: Highest First

      To whom it may concern,   I have a question regarding your Mouse MMP3 ELISA kit (ab100731).  On the datasheet regarding the kit, it states: “Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular protei...

      Read More

      ab100731 detects both the pro and mature form.   I hope that helps! Please let me know if you have any more questions.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"