MMP3 Mouse ELISA kit (ab100731)
- Product nameMMP3 Mouse ELISA kitSee all MMP3 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 10 pg/ml
- Range13.72 pg/ml - 10000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 117.4 106% - 130% Serum 120.6 101% - 140% Plasma 111.6 101% - 137%
- Species reactivityReacts with: Mouse
- Product overview
ab100731 MMP3 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP3 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for mouse MMP3 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MMP3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP3 bound. The StopSolution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 500X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent 5x concentrated buffer. ForStandard/Sample (serum/plasma samples/cell culture medium ) diluent. 1 x 15ml Detection Antibody MMP3: biotinylated anti-mouse MMP3 2 vials MMP3 Microplate (12 strips x 8 wells) coated with anti-mouse MMP3 1 unit MMP3 Standard Mouse (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
Contains 4 hemopexin-like domains.
- CHDS6Matrix metalloproteinase 3 Matrix metalloproteinase 3 preproprotein
- Matrix metalloproteinase-3MGC126102MGC126103MGC126104MMP 3MMP-3MMP3MMP3_HUMANProgelatinaseProteoglycanaseSL 1 SL-1SL1STMYSTMY1STR1 Stromelisin 1 Stromelysin 1Stromelysin 1 progelatinase Stromelysin-1Transin 1Transin-1
Our Abpromise guarantee covers the use of ab100731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for MMP3 Mouse ELISA kit (ab100731)
ab100731 has not yet been referenced specifically in any publications.