![Sandwich ELISA - MMP3 antibody [4B7.3] (ab38907) image](#MMP3-Primary-antibodies-ab38907-3.jpg)
![Western blot - MMP3 antibody [4B7.3] (ab38907) image](#ab38907_1.bmp)
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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP3 antibody [4B7.3]
See all MMP3 products (22) ...
Description
Mouse monoclonal [4B7.3] to MMP3
Specificity
The antibody is specific to MMP3 (Stromelysin 1). It binds to both the reduced (the pro form as well as active forms) and the non reduced proteins. The antibody does not cross react with the other Stromelysin forms (Stromelysin 2 and Stromelysin 3), nor to the other MMP family members (MMP1, MMP2, MMP9, etc.).
Tested applications
WB, Sandwich ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length protein of Human MMP3.
Positive control
Cell media from human chondrosarcoma (treated with TPA).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purification notes
The antibody has been affinity purified.
Clonality
Monoclonal
Clone number
4B7.3
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Detects a band of approximately 59 , 57 kDa (predicted molecular weight: 54 kDa).(Detects bands of approximately 59 and 57 kDa (the pro form) when used against the reduced protein. Dilution optimised using Chromogenic detection.)
sELISA: Use a concentration of 1 µg/ml Can be paired for Sandwich ELISA with Rabbit polyclonal to MMP3 - Whole molecule (ab38912). (For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab38912 as Detection.)
Target
Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP08254
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase 3 preproprotein antibody
see all
Database links
- Entrez Gene: 4314 Human
- Omim: 185250 Human
- SwissProt: P08254 Human
- Unigene: 375129 Human
Anti-MMP3 antibody [4B7.3] images:
Sandwich ELISA - MMP3 antibody [4B7.3] (ab38907)
![Sandwich ELISA - MMP3 antibody [4B7.3] (ab38907)](/ps/datasheet/images/38/ab38907/MMP3-Primary-antibodies-ab38907-3.jpg)
Standard curve for MMP3 (Analyte: ab39306) ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [4B7.3] to MMP3 (ab38907) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP3 - Whole molecule (ab38912) at 0.5µg/ml.
Western blot - MMP3 antibody [4B7.3] (ab38907)
![Western blot - MMP3 antibody [4B7.3] (ab38907)](/ps/datasheet/Images/38/ab38907/ab38907_1.bmp)
All lanes : Anti-MMP3 antibody [4B7.3] (ab38907) at 1/1000 dilution
Lane 1 : Human MMP3
Lane 2 : Cell media from human chondrosarcoma (treated with TPA)
Predicted band size : 54 kDa
When used against the reduced protein, the antibody identifies bands at 59 kDa and 57 kDa (the pro form).
References for Anti-MMP3 antibody [4B7.3] (ab38907)
This product has been referenced in:
- Hirata Het al. Role of secreted frizzled-related protein 3 in human renal cell carcinoma. Cancer Res 70:1896-905 (2010). WB; Human.Read more (PubMed: 20160027) »
- Pozgan Uet al. Expression and activity profiling of selected cysteine cathepsins and matrix metalloproteinases in synovial fluids from patients with rheumatoid arthritis and osteoarthritis. Biol Chem 391:571-9 (2010).Read more (PubMed: 20180636) »
See all 3 publications for this product
Publishing research using ab38907? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"