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Western blot - MMP3 antibody [4B7.3] (ab38907)
![Western blot - MMP3 antibody [4B7.3] (ab38907)](/ps/datasheet/images/ab38907_1.bmp)
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Product Name
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MMP3 antibody [4B7.3]
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See all MMP3 antibodies (23)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [4B7.3] to MMP3
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Immunogen
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Full length protein of Human MMP3.
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Reacts with
(species key)
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Hu
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Specificity
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The antibody is specific to MMP3 (Stromelysin 1). It binds to both the reduced (the pro form as well as active forms) and the non reduced proteins. The antibody does not cross react with the other Stromelysin forms (Stromelysin 2 and Stromelysin 3), nor to the other MMP family members (MMP1, MMP2, MMP9, etc.).
We have a range of domain specific antibodies for this target. For a full list please see all MMP3 antibodies
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: 1/1000 - 1/5000. Detects a band of approximately 59 and 57 kDa (predicted molecular weight: 54 kDa). Detects bands of approximately 59 and 57 kDa (the pro form) when used against the reduced protein. (Predicted molecular weight: 54 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Cell media from human chondrosarcoma (treated with TPA).
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Cellular localization
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Secreted; extracellular matrix
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Research areas
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Cardiovascular >> Atherosclerosis >> Thrombosis >> Other Cancer >> Tumor biomarkers >> Enzymes >> MMPs Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Relevance
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Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP3 is an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. MMP3 is reported to be implicated in rheumatoid arthritis and some forms of cancer.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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4B7.3
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Isotype
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IgG
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Storage buffer
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Preservative: 0.05% Sodium Azide Constituents: 50% Glycerol Material safety datasheets (MSDS) for this product: Glycerol MSDS Sodium Azide MSDS
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Purification notes
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The antibody has been affinity purified.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP3 antibody [4B7.3] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MMP3 antibody [4B7.3] images, references, products related to ab38907 and other tools.
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MMP3 antibody [4B7.3] images:
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Western blot - MMP3 antibody [4B7.3] (ab38907)
All lanes : MMP3 antibody [4B7.3] (ab38907) at 1/1000 dilution
Lane 1 : Human MMP3 Lane 2 : Cell media from human chondrosarcoma (treated with TPA)
Predicted band size : 54 kDa
When used against the reduced protein, the antibody identifies bands at 59 kDa and 57 kDa (the pro form).
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References for MMP3 antibody [4B7.3] (ab38907)
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This product has been used in:
Dixon JA et al. Mesenchymal cell transplantation and myocardial remodeling after myocardial infarction. Circulation 120:S220-9 (2009). PubMed: 19752372
If you publish research using ab38907 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MMP3
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MMP3 antibody [4B7.3] - more information
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Customer reviews (feedback) regarding MMP3 antibody [4B7.3] |
Customer FAQs regarding MMP3 antibody [4B7.3] |
Protocols for MMP3 antibody [4B7.3] |
Price and availability of products related to MMP3 antibody [4B7.3] |
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MMP3 antibody [4B7.3] - related products:
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MMP3 antibody [4B7.3] - other tools:
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Contact Abcam with a Technical Enquiry about MMP3 antibody [4B7.3] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"