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All lanes : Anti-MMP3 antibody - Aminoterminal end (ab38914) at 1/1000 dilution
Lane 1 : human MMP3
Lane 2 : cell medium from untreated human chondrosarcoma
Lane 3 : cell medium from TPA treated human chondrosarcoma
Predicted band size : 54 kDa
Observed band size : 47,57,59 kDa (why is the actual band size different from the predicted?)
Note: the 59 and 57 kD bands represent the pro forms of MMP3, while the 47 kD band represent its active form.
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