Anti-MMP3 antibody - Aminoterminal end (ab38914)

Overview

  • Product nameAnti-MMP3 antibody - Aminoterminal endSee all MMP3 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP3 - Aminoterminal end
  • Specificityab38914 recognises the Aminoterminal end of active enzyme MMP3 (stromelysin 1) and Stromelysin 2, but does not cross react with Stromelysin 3, nor to the other MMP family members (MMP 1, MMP 2, MMP 9, etc.).
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the aminoterminal end of the active form of human MMP3.

    (Peptide available as ab45251.)

Properties

Applications

Our Abpromise guarantee covers the use of ab38914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notesWB: 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. Predicted molecular weight: 54 kDa. But note that when used against the reduced protein, ab38914 identifies bands at 59 Kd and 57 Kd (the pro-form), as well as active forms. Also note that Stromelysin levels in quiescent cells and tissues are minimal, and stimulation or protein concentration is often needed to visualize the bands. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P08254 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase 3 preproprotein antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Progelatinase antibody
      • Proteoglycanase antibody
      • SL 1 antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelisin 1 antibody
      • Stromelysin 1 antibody
      • Stromelysin 1 progelatinase antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    Anti-MMP3 antibody - Aminoterminal end images

    • All lanes : Anti-MMP3 antibody - Aminoterminal end (ab38914) at 1/1000 dilution

      Lane 1 : human MMP3
      Lane 2 : cell medium from untreated human chondrosarcoma
      Lane 3 : cell medium from TPA treated human chondrosarcoma


      Predicted band size : 54 kDa
      Observed band size : 47,57,59 kDa (why is the actual band size different from the predicted?)

    References for Anti-MMP3 antibody - Aminoterminal end (ab38914)

    ab38914 has not yet been referenced specifically in any publications.

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