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Anti-MMP3 antibody [EP1186Y] - Carboxyterminal end (ab52915)

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Overview

Product name

Anti-MMP3 antibody [EP1186Y] - Carboxyterminal end
See all MMP3 products (22) ...

Description

Rabbit monoclonal [EP1186Y] to MMP3 - Carboxyterminal end

Tested applications

WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues near the C-terminus of human MMP3.

Positive control

Human gastric carcinoma tissue.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP1186Y

Isotype

IgG

Research areas

Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

  • Western blot - MMP3 antibody [EP1186Y] (ab52915)Western blot - MMP3 antibody [EP1186Y] (ab52915) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - MMP3 antibody [EP1186Y] (ab52915)Immunohistochemistry (Paraffin-embedded sections) - MMP3 antibody [EP1186Y] (ab52915) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/1000Detects a band...Read more →

    WB: 1/1000Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.(An...Read more →

    IHC-P: 1/100 - 1/250.(Antigen retrieval is recommended.)

  • ICC/IF

     ICC/IF: 1/100 - 1/250.

    ICC/IF: 1/100 - 1/250.

Application notes

Is unsuitable for ,Flow Cyt or IP.

Target

Function

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Sequence similarities

Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Cellular localization

Secreted > extracellular space > extracellular matrix.

Target information above from: UniProt accessionP08254 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CHDS6 antibody
  • Matrix metalloproteinase 3 antibody
  • Matrix metalloproteinase 3 preproprotein antibody
  • Matrix metalloproteinase-3 antibody
  • MGC126102 antibody
  • MGC126103 antibody
  • MGC126104 antibody
  • MMP 3 antibody
  • MMP-3 antibody
  • MMP3 antibody
  • MMP3_HUMAN antibody
  • Progelatinase antibody
  • Proteoglycanase antibody
  • SL 1 antibody
  • SL-1 antibody
  • SL1 antibody
  • STMY antibody
  • STMY1 antibody
  • STR1 antibody
  • Stromelisin 1 antibody
  • Stromelysin 1 antibody
  • Stromelysin 1 progelatinase antibody
  • Stromelysin-1 antibody
  • Transin 1 antibody
  • Transin-1 antibody
see all

Database links

see all

Anti-MMP3 antibody [EP1186Y] - Carboxyterminal end images:

  Western blot - MMP3 antibody [EP1186Y] (ab52915)

Western blot - MMP3 antibody [EP1186Y] (ab52915)

Anti-MMP3 antibody [EP1186Y] - Carboxyterminal end (ab52915) at 1/1000 dilution + Recombinant protein (0.5ng)

Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution

Predicted band size : 54 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - MMP3 antibody [EP1186Y] (ab52915)

Immunohistochemistry (Paraffin-embedded sections) - MMP3 antibody [EP1186Y] (ab52915)

Ab52915 (1:100) staining human MMP3 in human gastric carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

References for Anti-MMP3 antibody [EP1186Y] - Carboxyterminal end (ab52915)

This product has been referenced in:

  • Raaf Let al. Myocardial fibrosis and TGFB expression in hyperhomocysteinemic rats. Mol Cell Biochem 347:63-70 (2011). WB; Rat.Read more (PubMed: 20938722) »
  • Andersen Ket al. The metastasis-promoting protein S100A4 regulates mammary branching morphogenesis. Dev Biol 352:181-90 (2011). WB; Mouse.Read more (PubMed: 21195708) »

See all 3 publications for this product

Publishing research using ab52915? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"