Overview

  • Product nameAnti-MMP3 antibody [SPM293]See all MMP3 primary antibodies ...
  • Description
    Mouse monoclonal [SPM293] to MMP3
  • Tested applicationsICC/IF, ICC, WB, IHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human MMP3.
    Database link: P08254

  • Positive control
    • Conditioned, serum-free medium from TPA treated human Endometrial cells. Placenta. Bladder, breast, ovarian carcinomas.

Properties

Applications

Our Abpromise guarantee covers the use of ab17790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF
ICC
WB
IHC-P
  • Application notes
    ICC: Use at an assay dependent dilution.
    IF: Use at an assay dependent dilution.
    IHC-P: 1/10. Do not perform antigen retrieval.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 54 (proform) /44 (active form) kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P08254 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase 3 preproprotein antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Progelatinase antibody
      • Proteoglycanase antibody
      • SL 1 antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelisin 1 antibody
      • Stromelysin 1 antibody
      • Stromelysin 1 progelatinase antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    Anti-MMP3 antibody [SPM293] images

    • Anti-MMP3 antibody [SPM293] (ab17790) at 1 µg/ml + Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry using ab17790 on human placenta.

    References for Anti-MMP3 antibody [SPM293] (ab17790)

    This product has been referenced in:
    • Singh M  et al. Matrix metalloproteinases and their inhibitors and inducer in gestational trophoblastic diseases and normal placenta. Gynecol Oncol 122:178-82 (2011). IHC . Read more (PubMed: 21514631) »
    • O'Brien M  et al. Differential expression of the metalloproteinase MMP3 and the alpha5 integrin subunit in human myometrium at labour. Mol Hum Reprod 13:655-61 (2007). WB ; Human . Read more (PubMed: 17664256) »

    See all 2 Publications for this product

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