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ab39306 |
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Standard curve for MMP3 (Analyte: ab39306) ; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [4B7.3] to MMP3 (ab38907) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP3 - Whole molecule (ab38912) at 0.5µg/ml.
All lanes : Anti-MMP3 antibody - Whole molecule (ab38912) at 1/1000 dilution
Lane 1 : human MMP3
Lane 2 : untreated cell media from human chondrosarcoma
Lane 3 : TPA treated cell media from human chondrosarcoma
Predicted band size : 54 kDa
Observed band size : 47,50,57,59 kDa (why is the actual band size different from the predicted?)
Note: the bands at 59 and 57 kD represent the pro-form of MMP3. The bands at 50 and 47 kD represent the active form of MMP3.
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