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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP3 antibody
See all MMP3 products (22) ...
Description
Goat polyclonal to MMP3
Specificity
ab18898 recognises MMP-3.
Tested applications
IHC-Fr, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Horse, Human
Immunogen
Recombinant full length protein (Human)(Peptide available as ab53328.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - Anti-MMP3 antibody (ab18898)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab18898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr
IP
Application notes
IHC-Fr: Use at a concentration of 15 - 25 µg/ml.
IP: Use at a concentration of 10 - 20 µg/ml.
WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 54 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP08254
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase 3 preproprotein antibody
see all
Database links
- Entrez Gene: 4314 Human
- Entrez Gene: 17392 Mouse
- Entrez Gene: 171045 Rat
- Omim: 185250 Human
- SwissProt: Q28397 Horse
- SwissProt: P08254 Human
- SwissProt: P28862 Mouse
- SwissProt: P03957 Rat
see all
Anti-MMP3 antibody images:
Western blot - Anti-MMP3 antibody (ab18898)
Anti-MMP3 antibody (ab18898) at 1 µg/ml + MMP3 protein (Active) (ab96555) at 0.1 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 3 minutes
References for Anti-MMP3 antibody (ab18898)
This product has been referenced in:
- Clutterbuck ALet al. High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation. J Proteomics 74:704-15 (2011). WB; Horse.Read more (PubMed: 21354348) »
- McClain JAet al. Increased MMP-3 and CTGF expression during lipopolysaccharide-induced dopaminergic neurodegeneration. Neurosci Lett 460:27-31 (2009).Read more (PubMed: 19463894) »
See all 4 publications for this product
Publishing research using ab18898? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"