Overview

  • Product nameAnti-MMP3 antibody
    See all MMP3 primary antibodies
  • Description
    Goat polyclonal to MMP3
  • Specificityab18898 recognises MMP-3.
  • Tested applicationsIHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Human
  • Immunogen

    Recombinant full length protein (Human)(Peptide available as ab53328.)

Applications

Our Abpromise guarantee covers the use of ab18898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
IP
  • Application notesIHC-Fr: Use at a concentration of 15 - 25 µg/ml.
    IP: Use at a concentration of 10 - 20 µg/ml.
    WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 54 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Proteoglycanase antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelysin 1 antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    Anti-MMP3 antibody images

    • Anti-MMP3 antibody (ab18898) at 1 µg/ml + Recombinant human MMP3 protein (ab96555) at 0.1 µg

      Secondary
      Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 3 minutes

    References for Anti-MMP3 antibody (ab18898)

    This product has been referenced in:
    • Attia M  et al. Early Events of Overused Supraspinatus Tendons Involve Matrix Metalloproteinases and EMMPRIN/CD147 in the Absence of Inflammation. Am J Sports Med 41:908-17 (2013). WB ; Rat . Read more (PubMed: 23404084) »
    • Clutterbuck AL  et al. High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation. J Proteomics 74:704-15 (2011). WB ; Horse . Read more (PubMed: 21354348) »

    See all 5 Publications for this product

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    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to co...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Horse Tissue lysate - other (Cartilage explant culture secretome)
    Loading amount 50 µg
    Specification Cartilage explant culture secretome
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Ali Mobasheri

    Verified customer

    Submitted Jan 19 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Rat brain homogenate)
    Loading amount 60 µg
    Specification Rat brain homogenate
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Eduardo Candelario-Jalil

    Verified customer

    Submitted Jan 15 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"