Overview

  • Product nameAnti-MMP3 antibody
    See all MMP3 primary antibodies
  • Description
    Goat polyclonal to MMP3
  • Specificityab18898 recognises MMP-3.
  • Tested applicationsIHC-Fr, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Horse, Human
  • Immunogen

    Recombinant full length protein (Human)(Peptide available as ab53328.)

Properties

Applications

Our Abpromise guarantee covers the use of ab18898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
WB
IP
  • Application notesIHC-Fr: Use at a concentration of 15 - 25 µg/ml.
    IP: Use at a concentration of 10 - 20 µg/ml.
    WB: Use at a concentration of 1 - 4 µg/ml. Predicted molecular weight: 54 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase 3 preproprotein antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Progelatinase antibody
      • Proteoglycanase antibody
      • SL 1 antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelisin 1 antibody
      • Stromelysin 1 antibody
      • Stromelysin 1 progelatinase antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    Anti-MMP3 antibody images

    • Anti-MMP3 antibody (ab18898) at 1 µg/ml + Active human MMP3 full length protein (ab96555) at 0.1 µg

      Secondary
      Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 3 minutes

    References for Anti-MMP3 antibody (ab18898)

    This product has been referenced in:
    • Attia M  et al. Early Events of Overused Supraspinatus Tendons Involve Matrix Metalloproteinases and EMMPRIN/CD147 in the Absence of Inflammation. Am J Sports Med 41:908-17 (2013). WB ; Rat . Read more (PubMed: 23404084) »
    • Clutterbuck AL  et al. High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation. J Proteomics 74:704-15 (2011). WB ; Horse . Read more (PubMed: 21354348) »

    See all 5 Publications for this product

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    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to co...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Horse Tissue lysate - other (Cartilage explant culture secretome)
    Loading amount 50 µg
    Specification Cartilage explant culture secretome
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. Ali Mobasheri

    Verified customer

    Submitted Jan 19 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Rat brain homogenate)
    Loading amount 60 µg
    Specification Rat brain homogenate
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Eduardo Candelario-Jalil

    Verified customer

    Submitted Jan 15 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"