Anti-MMP3 antibody (ab53015)

Immunohistochemistry (Paraffin-embedded sections) - MMP3 antibody (ab53015)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP3 antibody at a dilution of 1/50. Left: without immunizing peptide; Right: with immunizing peptide.

Western blot - MMP3 antibody (ab53015)

All lanes : Anti-MMP3 antibody (ab53015) at 1/500 dilution

Lane 1 : Extracts from 293 cells, untreated.
Lane 2 : Extracts from 293 cells, treated with the immunising peptide.


Predicted band size : 54 kDa
Observed band size : 54 kDa

Immunocytochemistry/ Immunofluorescence - MMP3 antibody (ab53015)

ICC/IF image of ab53015 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53015, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry/ Immunofluorescence - MMP3 antibody (ab53015)

Top image: Isotype control
Middle image: Unstimulated cells
Bottom image: IL-1 beta stimulated cells (24 hours, 10 ng/ml)

ab53015 staining MMP3 in Human synovial fibroblasts by Immunocytochemistry/ Immunoflourescence. Cells were fixed in formaldehyde and permeabilized in methanol for 15 minutes at -20°C prior to blocking in 1% BSA for 1 hour at 25ºC. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours at 25ºC. The secondary antibody was Cy5®-conjugated Goat anti-Rabbit polyclonal, diluted 1/200.

This image is courtesy of an Abreview submitted by Torsten Lowin.