Overview

  • Product nameAnti-MMP3 antibody
    See all MMP3 primary antibodies
  • Description
    Rabbit polyclonal to MMP3
  • SpecificityMMP3 antibody detects endogenous levels of total MMP3 protein.
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from human MMP3 Peptide available as ab134501.

  • Positive control
    • Human breast carcinoma tissue, extracts from 293 cells.

Associated products

Applications

Our Abpromise guarantee covers the use of ab53015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human MMP3 peptide (ab134501).
ELISA 1/10000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHDS6 antibody
    • Matrix metalloproteinase 3 antibody
    • Matrix metalloproteinase-3 antibody
    • MGC126102 antibody
    • MGC126103 antibody
    • MGC126104 antibody
    • MMP 3 antibody
    • MMP-3 antibody
    • MMP3 antibody
    • MMP3_HUMAN antibody
    • Proteoglycanase antibody
    • SL-1 antibody
    • SL1 antibody
    • STMY antibody
    • STMY1 antibody
    • STR1 antibody
    • Stromelysin 1 antibody
    • Stromelysin-1 antibody
    • Transin 1 antibody
    • Transin-1 antibody
    see all

Anti-MMP3 antibody images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP3 antibody at a dilution of 1/50. Left: without immunizing peptide; Right: with immunizing peptide.Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP3 antibody at a dilution of 1/50. Left: without immunizing peptide; Right: with immunizing peptide.
  • All lanes : Anti-MMP3 antibody (ab53015) at 1/500 dilution

    Lane 1 : Extracts from 293 cells, untreated.
    Lane 2 : Extracts from 293 cells, treated with the immunising peptide.


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ICC/IF image of ab53015 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53015, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Top image: Isotype control Middle image: Unstimulated cells Bottom image: IL-1 beta stimulated cells (24 hours, 10 ng/ml)

    ab53015 staining MMP3 in Human synovial fibroblasts by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde and permeabilized in methanol for 15 minutes at -20°C prior to blocking in 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours at 25°C. The secondary antibody was Cy5®-conjugated Goat anti-Rabbit polyclonal, diluted 1/200.

    See Abreview

References for Anti-MMP3 antibody (ab53015)

This product has been referenced in:
  • Frankowski JC  et al. Detrimental role of the EP1 prostanoid receptor in blood-brain barrier damage following experimental ischemic stroke. Sci Rep 5:17956 (2015). WB ; Mouse, Rat . Read more (PubMed: 26648273) »
  • Ishrat T  et al. Low-dose candesartan enhances molecular mediators of neuroplasticity and subsequent functional recovery after ischemic stroke in rats. Mol Neurobiol 51:1542-53 (2015). WB ; Rat . Read more (PubMed: 25084762) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
Loading amount 100000 cells
Specification rat vascular smooth muscle cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2013

Thank you for your enquiry.

I have attached a copy of the MSDS for ab53015 MMP3 antibody. There is also now a link on the top right handside of the datasheet (SDS).

http://www.abcam.com/index.html?datasheet=53015 (or use the follo...

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Thank you for contacting Abcam regarding ab53015.


The immunogen region is XXX of human MMP3.



I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Thank you for your call today and for letting us know about the trouble with ab52915. I am sending a free of charge vial of ab53015, on the order ***. I have checked with the production lab, and the lysates used to test this antibody in Western blot we...

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Thank you for contacting us. We would suggest using PBS with 1% BSA as a diluting buffer. Please be advised that if you would like to store these antibodies at -20 or as recommended on the datasheet then we would recommend storing the antibodie...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (synovial fibroblasts)
Specification synovial fibroblasts
Fixative Formaldehyde
Permeabilization Yes - methanol, 15min -20 degrees
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Torsten Lowin

Verified customer

Submitted Feb 28 2011

Application Flow Cytometry
Sample Human Cell (synovial fibroblasts)
Specification synovial fibroblasts
Preparation Cell harvesting/tissue preparation method: cells detached with accutase (PAA)
Sample buffer: PBS with 1%BSA
Fixation Formaldehyde
Permeabilization Yes - methanol, 15min -20 degrees
Gating Strategy only one gate: fibroblasts
Username

Dr. Torsten Lowin

Verified customer

Submitted Feb 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Shoulder)
Specification Shoulder
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Vector Kit as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lymphoma)
Specification lymphoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0)
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"