MMP3 antibody (ab53015)

Product Name 

MMP3 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP3

Immunogen 

Synthetic peptide derived from human MMP3

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

MMP3 antibody detects endogenous levels of total MMP3 protein.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human breast carcinoma tissue, extracts from 293 cells.

Cellular localization 

Secreted; extracellular matrix

Research areas 

Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP3 is an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. MMP3 is reported to be implicated in rheumatoid arthritis and some forms of cancer.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for MMP3 antibody images, references, products related to ab53015 and other tools.

MMP3 antibody images:

References for MMP3 antibody (ab53015)

  This product has been used in: (two most recent references)

Thomay AA et al. Disruption of interleukin-1 signaling improves the quality of wound healing. Am J Pathol 174:2129-36 (2009). WB; Mouse. PubMed: 19389930

Echtermeyer F et al. Syndecan-4 regulates ADAMTS-5 activation and cartilage breakdown in osteoarthritis. Nat Med 15:1072-6 (2009). IHC-P; Mouse. PubMed: 19684582
If you publish research using ab53015 please let us know so that we can cite the reference on this datasheet.

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