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Read our guarantee »Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Anti-MMP3 antibody
See all MMP3 products (22) ...
Rabbit polyclonal to MMP3
MMP3 antibody detects endogenous levels of total MMP3 protein.
IHC-P, WB, ELISA, IHC-P, ICC/IFmore details
Reacts with
Mouse, Rat, Human
Synthetic peptide derived from human MMP3
Human breast carcinoma tissue, extracts from 293 cells.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Our Abpromise guarantee covers the use of ab53015 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000.Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ELISA: 1/10000
IHC-P: Use at an assay dependent concentration.
ICC/IF: Use a concentration of 5 µg/ml
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP08254
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Immunohistochemistry (Paraffin-embedded sections) - MMP3 antibody (ab53015)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP3 antibody at a dilution of 1/50. Left: without immunizing peptide; Right: with immunizing peptide.
Western blot - MMP3 antibody (ab53015)

All lanes : Anti-MMP3 antibody (ab53015) at 1/500 dilution
Lane 1 : Extracts from 293 cells, untreated.
Lane 2 : Extracts from 293 cells, treated with the immunising peptide.
Predicted band size : 54 kDa
Observed band size : 54 kDa
Immunocytochemistry/ Immunofluorescence - MMP3 antibody (ab53015)

ICC/IF image of ab53015 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53015, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - MMP3 antibody (ab53015)

Top image: Isotype control Middle image: Unstimulated cells Bottom image: IL-1 beta stimulated cells (24 hours, 10 ng/ml)
ab53015 staining MMP3 in Human synovial fibroblasts by Immunocytochemistry/ Immunoflourescence. Cells were fixed in formaldehyde and permeabilized in methanol for 15 minutes at -20°C prior to blocking in 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours at 25°C. The secondary antibody was Cy5®-conjugated Goat anti-Rabbit polyclonal, diluted 1/200.
This image is courtesy of an Abreview submitted by Torsten Lowin.
This product has been referenced in:
See all 5 publications for this product
Publishing research using ab53015? Please let us know so that we can cite the reference in this datasheet
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP3 antibody at a dilution of 1/50. Left: without immunizing peptide; Right: with immunizing peptide.

All lanes : Anti-MMP3 antibody (ab53015) at 1/500 dilution
Lane 1 : Extracts from 293 cells, untreated.
Lane 2 : Extracts from 293 cells, treated with the immunising peptide.
Predicted band size : 54 kDa
Observed band size : 54 kDa

ICC/IF image of ab53015 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53015, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Top image: Isotype control Middle image: Unstimulated cells Bottom image: IL-1 beta stimulated cells (24 hours, 10 ng/ml)
ab53015 staining MMP3 in Human synovial fibroblasts by Immunocytochemistry/ Immunoflourescence. Cells were fixed in formaldehyde and permeabilized in methanol for 15 minutes at -20°C prior to blocking in 1% BSA for 1 hour at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours at 25°C. The secondary antibody was Cy5®-conjugated Goat anti-Rabbit polyclonal, diluted 1/200.
This image is courtesy of an Abreview submitted by Torsten Lowin.

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