Overview

  • Product nameAnti-MMP3 antibodySee all MMP3 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP3
  • Tested applicationsWB, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MMP3.

    (Peptide available as ab73953.)

  • Positive control
    • This antibody gave a positive signal in the following Human Tissue lysates: Heart, Liver, Lung

Properties

Applications

Our Abpromise guarantee covers the use of ab63853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
IHC-P
  • Application notesIHC-P: 1/50 (See abreview).
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
    • Sequence similaritiesBelongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P08254 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • CHDS6 antibody
      • Matrix metalloproteinase 3 antibody
      • Matrix metalloproteinase 3 preproprotein antibody
      • Matrix metalloproteinase-3 antibody
      • MGC126102 antibody
      • MGC126103 antibody
      • MGC126104 antibody
      • MMP 3 antibody
      • MMP-3 antibody
      • MMP3 antibody
      • MMP3_HUMAN antibody
      • Progelatinase antibody
      • Proteoglycanase antibody
      • SL 1 antibody
      • SL-1 antibody
      • SL1 antibody
      • STMY antibody
      • STMY1 antibody
      • STR1 antibody
      • Stromelisin 1 antibody
      • Stromelysin 1 antibody
      • Stromelysin 1 progelatinase antibody
      • Stromelysin-1 antibody
      • Transin 1 antibody
      • Transin-1 antibody
      see all

    Anti-MMP3 antibody images

    • All lanes : Anti-MMP3 antibody (ab63853) at 1 µg/ml

      Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
      Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
      Lane 3 : Lung (Human) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 54 kDa
      Observed band size : 54 kDa


      Exposure time : 10 minutes
    • ab63853 staining MMP3 in Human breast carcinoma tissue sections by IHC-P (formaldehyde fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked by 5 minutes of peroxidase block then 10 minutes of protein block. Antigen retrieval was by heat mediation in Retrieval solution. Samples were incubated with primary antibody (1/50) for 45 minutes at 20°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.

      See Abreview

    References for Anti-MMP3 antibody (ab63853)

    ab63853 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (U251, NIH-3T3, Ms Ovary, PC12)
    Loading amount 20 µg
    Specification U251, NIH-3T3, Ms Ovary, PC12
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Feb 08 2010

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Breast carcinoma)
    Specification Breast carcinoma
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization No
    Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Jan 14 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"