MMP7 Human ELISA Kit (ab100608)
- Product nameMMP7 Human ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant
- Assay typeSandwich
- Sensitivity< 0.15 ng/ml
- Range0.137 ng/ml - 100 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 90.52 82% - 102%
- Species reactivityReacts with: Human
- Product overview
The ab100608 Human MMP7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP7 in cell culture supernatants. This assay employs an antibody specific for human MMP7 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MMP7 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 500X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent 5x concentrated buffer. ForStandard/Sample (serum/plasma samples/cell culture medium/urine) diluent. 1 x 15ml Detection Antibody MMP-7: biotinylated anti-human MMP-7 2 vials MMP 7 Microplate (12 strips x 8 wells) coated with anti-human MMP-7 1 unit MMP 7 Standard Human(recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- MatrilysinMatrinMatrix metalloproteinase 7
- Matrix metalloproteinase-7MMP-7MMP7MMP7_HUMANMPSL1Pump 1 proteasePump-1 proteasePUMP1Uterine metalloproteinase
Our Abpromise guarantee covers the use of ab100608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for MMP7 Human ELISA Kit (ab100608)
ab100608 has not yet been referenced specifically in any publications.