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Overview

  • Product nameMMP7 Human ELISA Kit
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant
  • Assay typeSandwich
  • Sensitivity
    < 0.15 ng/ml
  • Range
    0.137 ng/ml - 100 ng/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 90.52 82% - 102%

  • Species reactivity
    Reacts with: Human
  • Product overview

    The ab100608 Human MMP7 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP7 in cell culture supernatants. This assay employs an antibody specific for human MMP7 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MMP7 antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • FunctionDegrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
  • Sequence similaritiesBelongs to the peptidase M10A family.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.

    • Target information above from: UniProt accession P09237 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        MatrilysinMatrinMatrix metalloproteinase 7
        Matrix metalloproteinase-7MMP-7MMP7MMP7_HUMANMPSL1Pump 1 proteasePump-1 proteasePUMP1Uterine metalloproteinase
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100608 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      MMP7 Human ELISA Kit images

      • ELISA - MMP7 Human ELISA Kit (ab100608)
        ELISA - MMP7 Human ELISA Kit (ab100608)

      Protocols

      References for MMP7 Human ELISA Kit (ab100608)

      ab100608 has not yet been referenced specifically in any publications.

      Publishing research using ab100608 ? Please let us know so that we can cite the reference in this datasheet

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      Dear xxxx,
      I forwarded you proporsal to out customer.
      Please see here below her answer:

      I now have further information to share from the lab in regards to the MMP7 Human ELISA Kit (ab100608).

      The lab have further confirmed that the results obtained with the lot numbers GR88563 & GR98815 have performed as expected: standard curve...

      Read More

      Dear Karin,
      please see here below our customer's answers:

      Here our responses, keeping in mind that we carefully followed the instructions provided with the kit, and that we are not naive to immunoenzymatic assays.

      1. How was the...

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      I am sorry for the delay in getting back to you. I now have some further input form the lab regarding the MMP7 Human ELISA Kit (ab100608) your customer has been having some difficulty with.

      The first lot tested does contain good linearity and lo...

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      Dear All,
      a customer of ours has bought 4 kit of ab100608 in 2 different orders (2 kits with our xxxx and 2 kits with our xxxx).
      She tested 3 kits and she has some problem with the standard curve. Please see attachment.

      Can you help h...

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      Thank you for contacting us.

      I am sorry to hear your customer has been experiencing difficulties with the MMP7 Human ELISA Kit (ab100608). We take product complaints very seriously, and investigate every product that we feel may not be performi...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"