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- Proteins and Peptides
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Product name
Anti-MMP7 antibody - Aminoterminal end
See all MMP7 products (12) ...
Description
Rabbit polyclonal to MMP7 - Aminoterminal end
Specificity
ab39001 recognizes the aminoterminal end of the active enzyme MMP7. It does not cross react with the other MMP family members.
Tested applications
Cross reactivity
Reacts with
Human, Pig
Immunogen
Synthetic peptide corresponding to the aminoterminal end of human MMP7 active enzyme.
(Peptide available as ab410 49.)
Positive control
human MMP7, cell media from pig kidney, epithelial morphology (IL1 alpha treated).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
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Western blot - MMP7 antibody (ab39001)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 when using colormetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 29 kDa. When used against the reduced protein, ab39001 identifies bands at about 28 Kd, and 18Kd (the pro-form and active forms). Note: MMP7 does not appear to be produced by most normal quiescent cells, but treatment of many cell types with the phorbol ester TPA stimulates its production. Because of the low protein levels produced (pg/mL) concentration of cell culture media is often required to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP09237
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrilysin antibody
- Matrilysin uterine antibody
- Matrin antibody
see all
Database links
- Entrez Gene: 4316 Human
- Omim: 178990 Human
- SwissProt: P09237 Human
- Unigene: 2256 Human
Anti-MMP7 antibody - Aminoterminal end images:
Western blot - MMP7 antibody (ab39001)
All lanes : Anti-MMP7 antibody - Aminoterminal end (ab39001) at 1/1000 dilution
Lane 1 : human MMP7
Lane 2 : cell media from pig kidney, epithelial morphology (IL1 alpha treated).
Predicted band size : 29 kDa
Observed band size : 22,24,29 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-MMP7 antibody - Aminoterminal end (ab39001)
ab39001 has not yet been referenced specifically in any publications.
Publishing research using ab39001? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"