|
|
|
|
Western blot - MMP7 antibody (ab39984)
(enlarge)
|
|
|
|
|
Product Name
|
MMP7 antibody
|
| |
See all MMP7 antibodies (10)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Goat polyclonal to MMP7
|
|
Immunogen
|
Synthetic peptide: QKLYGKRSNSRKK, corresponding to C terminal amino acids 255/267 of Human MMP7
BLAST 'QKLYGKRSNSRKK' with  or 
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:64,000
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 28 kDa. (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Human Breast lysate
|
|
Cellular localization
|
Extracellular space
|
|
Research areas
|
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
|
|
Relevance
|
Matrix Metalloproteinases (MMPs) belong to a family of proteases that are essential for the breakdown of extracellular matrix (ECM). They are thus important in apoptosis, tumor cell growth, invasion, and metastasis, as well as angiogenesis and wound healing. Most MMPs contain common domain structures that include a signal sequence, a propeptide, a catalytic domain, and a hemopexin like (Hpx) domain. MMP7, also known as Matrilysin, is responsible for hydrolyzing proteoglycans and ECM glycoproteins. It is expressed in normal cells, specifically glandular epithelial cells. MMP7, is thought to play an important role in the early steps of tumor progression. Expression of MMP2, MMP7 and MMP11 is greater in pancreatic carcinoma than in normal pancreas.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Goat
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MMP7 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for MMP7 antibody images, references, products related to ab39984 and other tools.
|
| |
MMP7 antibody images:
|
|
|
| |
| |
|
Search PubMed (MEDLINE) for references to MMP7
|
| |
MMP7 antibody - more information
|
Customer reviews (feedback) regarding MMP7 antibody |
Customer FAQs regarding MMP7 antibody |
Protocols for MMP7 antibody |
Price and availability of products related to MMP7 antibody |
| |
MMP7 antibody - related products:
|
|
|
| |
MMP7 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about MMP7 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
Western blot - MMP7 antibody (ab39984)