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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab4044? Please let us know so that we can cite the reference in this datasheet
ab4044 has been referenced in 1 publications.
- Hoeth M et al. The Transcription Factor SOX18 Regulates the Expression of Matrix Metalloproteinase 7 and Guidance Molecules in Human Endothelial Cells. PLoS One 7:e30982 (2012). IHC-P; Human. PubMed: 22292085
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Frozen sections) - MMP7 antibody (ab4044)
ab4044 staining MMP7 in Human stomach tissue sections by IHC-Fr (Immunohistochemistry - Froxen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 1 hour at 25ºC. Samples were incubated with primary antibody 1/50 in blocking buffer for 1 hour at 25ºC. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP7 antibody (ab4044)
ab4044 at 1/50 dilution staining MMP7 in human stomach tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were formaldehyde fixed prior to blocking in 5% serum for 1 hour at 23ºC and then incubated with ab4044 for 1 hour at 23ºC. A HRP conjugated goat polyclonal to rabbit Ig was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - MMP7 antibody (ab4044)
ab4044 staining MMP7 in Human AGS Gastric Carcinoma cells by immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilized in TBS (0.025% Triton-X 100) and blocked in 5% serum for 1 hour at 23°C. The primary was diluted 1/50 and incubated with sample for 1 hour at 23°C. An Alexa Fluor® 568 conjugated Goat polyclonal to Rabbit IgG at 1/500 dilution, was used as secondary antibody. The primary antibody shows red staining of MMP7 in image whilst beta actin was stained green with Alexa Fluor® 488.
This image is courtesy of an anonymous Abreview.
Western blot - MMP7 antibody (ab4044)
Anti-MMP7 antibody (ab4044) at 1/1000 dilution (in blocking buffer for 16 hours at 4°C) + Whole cell lysate of Human AGS gastric carcinoma cells at 20 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/1000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
Blocking Step : 5% BSA for 1 hour at 23°C
This image is courtesy of an anonymous Abreview
MMP7 antibody for IHC-P in Human (4044)
MMP7 antibody for IHC-P in Human (4044)
MMP7 antibody for IHC-Fr in Human (4044)
MMP7 antibody for Western blot in Human (4044)
MMP7 antibody for Western blot in Mouse (4044)
MMP7 antibody for Immunocytochemistry in Human (4044)
MMP7 antibody for ICC/IF in Human (4044)
MMP7 antibody for Immunocytochemistry/ Immunofluorescence in Mouse (4044)
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