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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP7 antibody
See all MMP7 products (12) ...
Description
Rabbit polyclonal to MMP7
Specificity
This antibody reacts with the 28 kDa protein (pro form) and 18 kDa (active form) of MMP 7.
Tested applications
WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A synthetic peptide from the human matrilysin (PUMP-1).
Positive control
Placenta.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: 1/50
ICC: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
Target
Function
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP09237
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrilysin antibody
- Matrilysin uterine antibody
- Matrin antibody
see all
Database links
- Entrez Gene: 4316 Human
- Entrez Gene: 17393 Mouse
- Omim: 178990 Human
- SwissProt: P09237 Human
- SwissProt: Q10738 Mouse
- Unigene: 2256 Human
- Unigene: 4825 Mouse
Anti-MMP7 antibody images:
Immunohistochemistry (Frozen sections) - MMP7 antibody (ab4044)
ab4044 staining MMP7 in Human stomach tissue sections by IHC-Fr (Immunohistochemistry - Froxen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody 1/50 in blocking buffer for 1 hour at 25°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP7 antibody (ab4044)
ab4044 at 1/50 dilution staining MMP7 in human stomach tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were formaldehyde fixed prior to blocking in 5% serum for 1 hour at 23°C and then incubated with ab4044 for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit Ig was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Immunocytochemistry/ Immunofluorescence - MMP7 antibody (ab4044)
ab4044 staining MMP7 in Human AGS Gastric Carcinoma cells by immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilized in TBS (0.025% Triton-X 100) and blocked in 5% serum for 1 hour at 23°C. The primary was diluted 1/50 and incubated with sample for 1 hour at 23°C. An Alexa Fluor® 568 conjugated Goat polyclonal to Rabbit IgG at 1/500 dilution, was used as secondary antibody. The primary antibody shows red staining of MMP7 in image whilst beta actin was stained green with Alexa Fluor® 488.
This image is courtesy of an anonymous Abreview.
Western blot - MMP7 antibody (ab4044)
Anti-MMP7 antibody (ab4044) at 1/1000 dilution (in blocking buffer for 16 hours at 4°C) + Whole cell lysate of Human AGS gastric carcinoma cells at 20 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/1000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
Blocking Step : 5% BSA for 1 hour at 23°C
This image is courtesy of an anonymous Abreview
References for Anti-MMP7 antibody (ab4044)
ab4044 has not yet been referenced specifically in any publications.
Publishing research using ab4044? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"