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Our Abpromise guarantee covers the use of ab4044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
ab4044 staining MMP7 in Human AGS Gastric Carcinoma cells by immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilized in TBS (0.025% Triton-X 100) and blocked in 5% serum for 1 hour at 23°C. The primary was diluted 1/50 and incubated with sample for 1 hour at 23°C. An Alexa Fluor® 568 conjugated Goat polyclonal to Rabbit IgG at 1/500 dilution, was used as secondary antibody. The primary antibody shows red staining of MMP7 in image whilst beta actin was stained green with Alexa Fluor® 488.
This image is courtesy of an anonymous Abreview
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