Overview

  • Product nameAnti-MMP7 antibodySee all MMP7 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP7
  • SpecificityThis antibody reacts with the 28 kDa protein (pro form) and 18 kDa (active form) of MMP 7.
  • Tested applicationsWB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human MMP7 aa 1-267.

  • Positive control
    • Placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab4044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution.
IHC-Fr 1/50.
ICC Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • FunctionDegrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
  • Sequence similaritiesBelongs to the peptidase M10A family.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrilysin antibody
    • Matrilysin uterine antibody
    • Matrin antibody
    • Matrix Metalloproteinase 7 antibody
    • Matrix metalloproteinase-7 antibody
    • MMP 7 antibody
    • MMP-7 antibody
    • MMP7 antibody
    • MMP7_HUMAN antibody
    • MPSL1 antibody
    • PUMP 1 antibody
    • Pump 1 protease antibody
    • Pump-1 protease antibody
    • PUMP1 antibody
    • Uterine Matrilysin antibody
    • Uterine metalloproteinase antibody
    see all

Anti-MMP7 antibody images

  • ab4044 staining MMP7 in Human stomach tissue sections by IHC-Fr (Immunohistochemistry - Froxen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody 1/50 in blocking buffer for 1 hour at 25°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.

    See Abreview

  • ab4044 at 1/50 dilution staining MMP7 in human stomach tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were formaldehyde fixed prior to blocking in 5% serum for 1 hour at 23°C and then incubated with ab4044 for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit Ig was used as the secondary antibody.

    See Abreview

  • ab4044 staining MMP7 in Human AGS Gastric Carcinoma cells by immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilized in TBS (0.025% Triton-X 100) and blocked in 5% serum for 1 hour at 23°C. The primary was diluted 1/50 and incubated with sample for 1 hour at 23°C. An Alexa Fluor® 568 conjugated Goat polyclonal to Rabbit IgG at 1/500 dilution, was used as secondary antibody. The primary antibody shows red staining of MMP7 in image whilst beta actin was stained green with Alexa Fluor® 488.

    See Abreview

  • Anti-MMP7 antibody (ab4044) at 1/1000 dilution (in blocking buffer for 16 hours at 4°C) + Whole cell lysate of Human AGS gastric carcinoma cells at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/1000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human non-small-cell lung cancer tissue staining MMP7 with ab4044.

    Antigen retrieval was performed by placing the sections in 0.01 M citrate buffer (pH 6) and heating in a microwave oven. Samples were incubated with primary antibody (1/15) overnight at 4°C. Staining was detected using an IHC substrate kit.

References for Anti-MMP7 antibody (ab4044)

This product has been referenced in:
  • Erbel C  et al. CXCL4-induced plaque macrophages can be specifically identified by co-expression of MMP7+S100A8+ in vitro and in vivo. Innate Immun N/A:N/A (2014). Human . Read more (PubMed: 24663337) »
  • Phillips KL  et al. Interleukin-1 receptor antagonist deficient mice provide insights into pathogenesis of human intervertebral disc degeneration. Ann Rheum Dis N/A:N/A (2013). IHC ; Mouse . Read more (PubMed: 23396662) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
Loading amount 100000 cells
Specification rat vascular smooth muscle cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2013

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab4044.

To check the status of the order please contact ou...

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We have not seen a band at around 60-70 kDa for MMP7.



This may be a problem with the primary antibody. If you have ordered this vial of ab4044 within the last 6 months or so I would be happy to offer a replacement, credit or refund...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this MMP7 antibody.

The details you have kindly provided will provide us with...

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I have processed your request to receive ab5706 as a replacement for ab4044. Your new order number is *****. If there is anything else I can help you with, please let me know.

I am sorry that the protocol tips did not prove to be successful. I can either send you a new vial of the antibody and you could continue testing with that. Or. I could send you a different antibody to MMP7, I was thinking that ab5706, would be a suita...

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I am sorry that you are having issues with one of our antibodies in western blot. I have looked over the information and the image that you sent and you done an extensive amount of troubleshooting already. As the bands that you are seeing are about 60-...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3 Mouse Fibroblasts)
Specification NIH3T3 Mouse Fibroblasts
Fixative Formaldehyde
Permeabilization Yes - 0.025% Triton-X in TBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jan 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Intestine)
Loading amount 20 µg
Specification Intestine
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 23 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (AGS Human gastric carcinoma cells)
Loading amount 20 µg
Specification AGS Human gastric carcinoma cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"