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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP7 antibody
See all MMP7 products (12) ...
Description
Rabbit polyclonal to MMP7
Specificity
This antibody specifically recognises MMP7, and does not cross react with MMP1, 2, 3, 8, 9, 10 or 16.
Tested applications
WB, ELISA, IP, IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide corresponding to C-Terminus of MMP7 (Human).
Positive control
PMA stimulated A431 whole cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Proclin
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
IHC-Fr: Use at an assay dependent dilution (PMID 18499699).
IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use 3-5µg for 106 cells.
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 28 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP09237
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrilysin antibody
- Matrilysin uterine antibody
- Matrin antibody
see all
Database links
- Entrez Gene: 4316 Human
- Entrez Gene: 17393 Mouse
- Entrez Gene: 25335 Rat
- Omim: 178990 Human
- SwissProt: P09237 Human
- SwissProt: Q10738 Mouse
- SwissProt: P50280 Rat
- Unigene: 2256 Human
see all
Anti-MMP7 antibody images:
Western blot - MMP7 antibody (ab5706)
The whole cell lysate derived from PMA-stimulated A431 was probed with rabbit anti MMP7 at 1/500 (Lane A) or by pre-absorption antibody with immunizing peptides (Lane B).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-MMP7 antibody(ab5706)
ab5706 (2µg/ml) staining MMP7 in human kidney cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei within proximal convoluted tubules.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-MMP7 antibody (ab5706)
This product has been referenced in:
- Lozito TP & Tuan RS Mesenchymal stem cells inhibit both endogenous and exogenous MMPs via secreted TIMPs. J Cell Physiol 226:385-96 (2011). WB; Human.Read more (PubMed: 20665704) »
- Bernert Bet al. Hyaluronan Synthase 2 (HAS2) Promotes Breast Cancer Cell Invasion by Suppression of Tissue Metalloproteinase Inhibitor 1 (TIMP-1). J Biol Chem 286:42349-59 (2011). WB.Read more (PubMed: 22016393) »
See all 4 publications for this product
Publishing research using ab5706? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"