MMP7 antibody (ab5706)

Product Name 

MMP7 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MMP7

Immunogen 

Synthetic peptide corresponding to C-Terminus of MMP7 (Human).

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Specificity 

This antibody specifically recognises MMP7, and does not cross react with MMP1, 2, 3, 8, 9, 10 or 16.

Tested applications 
(see key)

ELISA, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: Use at a concentration of 0.1 - 1.0 µg/ml.
IHC-Fr: Use at an assay dependent dilution (PMID 18499699).
IP: Use 3-5µg for 10⁶ cells.
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects a band of approximately 28 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

PMA stimulated A431 whole cell lysate.

Cellular localization 

Extracellular space

Research areas 

Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

Relevance 

Matrix Metalloproteinases (MMPs) belong to a family of proteases that are essential for the breakdown of extracellular matrix (ECM). They are thus important in apoptosis, tumor cell growth, invasion, and metastasis, as well as angiogenesis and wound healing. Most MMPs contain common domain structures that include a signal sequence, a propeptide, a catalytic domain, and a hemopexin like (Hpx) domain. MMP7, also known as Matrilysin, is responsible for hydrolyzing proteoglycans and ECM glycoproteins. It is expressed in normal cells, specifically glandular epithelial cells. MMP7, is thought to play an important role in the early steps of tumor progression. Expression of MMP2, MMP7 and MMP11 is greater in pancreatic carcinoma than in normal pancreas.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Proclin
Constituents: PBS, pH 7.4

Form 

Liquid

Concentration 

0.200 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for MMP7 antibody images, references, products related to ab5706 and other tools.

MMP7 antibody images:

References for MMP7 antibody (ab5706)

  This product has been used in:

Guillen-Ahlers H et al. Sulindac treatment alters collagen and matrilysin expression in adenomas of ApcMin/+ mice. Carcinogenesis 29:1421-7 (2008). IHC-Fr; Mouse. PubMed: 18499699

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