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- Secondary antibodies
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Product name
Anti-MMP7 antibody
See all MMP7 products (12) ...
Description
Sheep polyclonal to MMP7
Specificity
This product has been shown to be specific by Western blotting.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
The immunogen used was a 15 amino acid synthetic peptide, selected from the C-terminus of human MMP-7, which was chosen tominimise homologies with MMPs and other proteins. The peptide was synthesised as a multi-antigenic peptide (MAP), in order to avoidthe problems inherent with the use of carrier proteins.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab8483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
This product is suitable for use in Western blotting. It has been evaluated using reducing conditions and a 10-20% SDS-PAGE gel. Thefollowing dilutions should be used as guidelines for determining those appropriate for the users system.
Use at 1/1000 (Antigen: 100ng recombinant human MMP-7)
Suitability for use in immunohistochemical procedures and enzyme-linked immunosorbent assays has not been assessed but use insuch assays should not necessarily be excluded.
Target
Function
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP09237
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrilysin antibody
- Matrilysin uterine antibody
- Matrin antibody
see all
Database links
- Entrez Gene: 4316 Human
- Omim: 178990 Human
- SwissProt: P09237 Human
- Unigene: 2256 Human
References for Anti-MMP7 antibody (ab8483)
ab8483 has not yet been referenced specifically in any publications.
Publishing research using ab8483? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"