Overview

  • Product nameAnti-MMP7 antibodySee all MMP7 primary antibodies ...
  • Description
    Rabbit polyclonal to MMP7
  • Tested applicationsIHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Sheep, Horse, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human MMP7.

    (Peptide available as ab95052.)

  • Positive control
    • This antibody gave a positive signal in A431 whole cell lysate and in the following human tissue lysates; Placenta; Liver; Kidney; Small Intestine.

Properties

Applications

Our Abpromise guarantee covers the use of ab85144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Can be blocked with Human MMP7 peptide (ab95052). Also detects a band at approximately 22 kDa.

Target

  • FunctionDegrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
  • Sequence similaritiesBelongs to the peptidase M10A family.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • Matrilysin antibody
    • Matrin antibody
    • Matrix Metalloproteinase 7 antibody
    • Matrix metalloproteinase-7 antibody
    • MMP 7 antibody
    • MMP-7 antibody
    • MMP7 antibody
    • MMP7_HUMAN antibody
    • MPSL1 antibody
    • PUMP 1 antibody
    • Pump 1 protease antibody
    • Pump-1 protease antibody
    • PUMP1 antibody
    • Uterine matrilysin antibody
    • Uterine metalloproteinase antibody
    see all

Anti-MMP7 antibody images

  • All lanes : Anti-MMP7 antibody (ab85144) at 1 µg/ml

    Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
    Lane 3 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
    Lane 4 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
    Lane 5 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 22,29 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,40 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes
  • IHC image of MMP7 staining in human pancreatic cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85144, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References for Anti-MMP7 antibody (ab85144)

ab85144 has not yet been referenced specifically in any publications.

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