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Product name
Anti-MMP7 antibody
See all MMP7 products (12) ...
Description
Rabbit polyclonal to MMP7
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Sheep, Horse, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human MMP7.
(Peptide available as ab950 52.)
Positive control
This antibody gave a positive signal in A431 whole cell lysate and in the following human tissue lysates; Placenta; Liver; Kidney; Small Intestine.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab85144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/ml.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).Also detects a band at approximately 22 kDa.
Target
Function
Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Sequence similarities
Belongs to the peptidase M10A family.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP09237
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Matrilysin antibody
- Matrin antibody
- Matrix Metalloproteinase 7 antibody
see all
Database links
- Entrez Gene: 4316 Human
- Omim: 178990 Human
- SwissProt: P09237 Human
- Unigene: 2256 Human
Anti-MMP7 antibody images:
Western blot - MMP7 antibody (ab85144)
All lanes : Anti-MMP7 antibody (ab85144) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lane 3 :
Lane 4 :
Lane 5 :
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 29 kDa
Observed band size : 22,29 kDa (why is the actual band size different from the predicted?)
Additional bands at : 200 kDa,40 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 minutes
MMP7 protein contains both a 17-amino acid signal sequence and an activation peptide (77 amino-acids) (Swissprot). If both were removed, the expected MW would be around 20-kDa. We hypothesise that the band at approximately 20-kDa, detected in A431 whole cell lysate, and in human liver, kidney and small intestine tissue lysates, is the cleaved form of MMP7. The band at approximately 29-kDa in human placenta tissue lysate is probably the full length protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP7 antibody (ab85144)
IHC image of MMP7 staining in human pancreatic cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85144, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
References for Anti-MMP7 antibody (ab85144)
ab85144 has not yet been referenced specifically in any publications.
Publishing research using ab85144? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"