MMP8 Rat ELISA Kit (ab100779)
- Product nameMMP8 Rat ELISA KitSee all MMP8 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 50 pg/ml
- Range0.05 ng/ml - 40 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.42 83% - 108% Serum 109.78 99% - 118% Plasma 107.6 98% - 118%
- Species reactivityReacts with: Rat
- Product overview
ab100779 MMP8 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP8 in serum, plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended), cell culture supernatants and urine. This assay employs an antibody specific for MMP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat MMP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A . diluent buffer For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody MMP8 : biotinylated anti-rat MMP8 2 vials HRP-Streptavidin Concentrate 160X Item G 1 x 200µl MMP8 Microplate (12 strips x 8 wells) coated with anti-rat MMP8 1 unit MMP8 Standard Rat (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
Contains 4 hemopexin-like domains.
- BB138268CLG 1CLG1
- Collagenase 1Collagenase 1 neutrophil HNCMatrix metallopeptidase 8Matrix metalloproteinase 8 (neutrophil collagenase)Matrix metalloproteinase-8MMP 8MMP-8Mmp8MMP8_HUMANNeutrophil collagenasePMNL CLPMNL collagenasePMNL-CL
Our Abpromise guarantee covers the use of ab100779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for MMP8 Rat ELISA Kit (ab100779)
ab100779 has not yet been referenced specifically in any publications.