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Overview

  • Product nameMMP8 Rat ELISA KitSee all MMP8 kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 50 pg/ml
  • Range
    0.05 ng/ml - 40 ng/ml
  • Recovery

    100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.42 83% - 108%
    Serum 109.78 99% - 118%
    Plasma 107.6 98% - 118%

  • Species reactivity
    Reacts with: Rat
  • Product overview

    ab100779 MMP8 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP8 in serum, plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended), cell culture supernatants and urine. This assay employs an antibody specific for MMP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat MMP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details

Properties

  • Storage instructionsStore at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    20X Wash Buffer Concentrate Item B 1 x 25ml
    Assay Diluent A . diluent buffer For Standard/Sample (serum/plasma) diluent. 1 x 30ml
    Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml
    Detection Antibody MMP8 : biotinylated anti-rat MMP8 2 vials
    HRP-Streptavidin Concentrate 160X Item G 1 x 200µl
    MMP8 Microplate (12 strips x 8 wells) coated with anti-rat MMP8 1 unit
    MMP8 Standard Rat (recombinant) 2 vials
    Stop Solution Item I 1 x 8ml
    TMB One-Step Substrate Reagent Item H 1 x 12ml
  • Research Areas
  • FunctionCan degrade fibrillar type I, II, and III collagens.
  • Tissue specificityNeutrophils.
  • Sequence similaritiesBelongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localizationCytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.

    • Target information above from: UniProt accession P22894 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        BB138268CLG 1CLG1
        Collagenase 1Collagenase 1 neutrophil HNCMatrix metallopeptidase 8Matrix metalloproteinase 8 (neutrophil collagenase)Matrix metalloproteinase-8MMP 8MMP-8Mmp8MMP8_HUMANNeutrophil collagenasePMNL CLPMNL collagenasePMNL-CL
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100779 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      MMP8 Rat ELISA Kit images

      • ELISA - MMP8 Rat ELISA Kit (ab100779)
        ELISA - MMP8 Rat ELISA Kit (ab100779)

      Protocols

      References for MMP8 Rat ELISA Kit (ab100779)

      ab100779 has not yet been referenced specifically in any publications.

      Publishing research using ab100779 ? Please let us know so that we can cite the reference in this datasheet

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      Submit an Abreview for ab100779 Submit a Question for ab100779

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      ab100779, ab100609: can cross-react withmouse?
      info on testing discount program

      Thank you for your patience.

      The lab let me know the following:

      ab100609 : Cross reactivity with mouse is unknown. If it helps, the capture antibody is monoclonal and the detection antibody is polyclonal.

      ab100779 : Untested...

      Read More

      ab100779, ab100609: can cross-react withmouse?
      info on testing discount program

      Thank you for contacting us.

      I have contacted the lab regarding the cross-reactivity of the 2 kits and will let you know what I find out.

      As for the testing discount program, we have the following general information:

      For UN...

      Read More

      Dear,

      If your kit below can measure activity MMP-8


      MMP8 Rat ELISA Kit - 1 x 96 Well Plate (ab100779)

      Thank you for your enquiry.

      ab100779 does not measure MMP8 activity. It is a sandwich ELISA assay that just quantitates amount of protein present.

      I hope this is helpful. Please contact me again if you have any further questions.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"