![Sandwich ELISA - MMP8 antibody [MM0023-7A11] (ab56303) image](#MMP8-Primary-antibodies-ab56303-5.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP8 antibody [MM0023-7A11] (ab56303) image](#MMP8-Primary-antibodies-ab56303-1.jpg)
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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP8 antibody [MM0023-7A11]
See all MMP8 products (12) ...
Description
Mouse monoclonal [MM0023-7A11] to MMP8
Specificity
This antibody recognizes human MMP8 in western blot without cross reactivity with other MMPs.
Tested applications
IHC-P, Sandwich ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human)
Positive control
Human recombinant MMP8
Properties
Form
Lyophilised:
Reconstitute with 0.5ml sterile PBS.
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
The IgG fraction of the tissue culture supernatant was purified then lyophilized from a 0.2 µm filtered PBS solution.
Clonality
Monoclonal
Clone number
MM0023-7A11
Isotype
IgG2
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/200.
sELISA: Use a concentration of 5 µg/ml Can be paired for Sandwich ELISA with Rabbit polyclonal to MMP8 - Hinge region (ab38994). (For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab38994 as Detection.)
WB: 1/400 - 1/1000.Predicted molecular weight: 53 kDa.
Target
Function
Can degrade fibrillar type I, II, and III collagens.
Tissue specificity
Neutrophils.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
Target information above from: UniProt accessionP22894
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CLG 1 antibody
- CLG1 antibody
- Collagenase 1 antibody
see all
Database links
- Entrez Gene: 4317 Human
- Omim: 120355 Human
- SwissProt: P22894 Human
- Unigene: 161839 Human
Anti-MMP8 antibody [MM0023-7A11] images:
Sandwich ELISA - MMP8 antibody [MM0023-7A11] (ab56303)
![Sandwich ELISA - MMP8 antibody [MM0023-7A11] (ab56303)](/ps/datasheet/images/56/ab56303/MMP8-Primary-antibodies-ab56303-5.jpg)
Standard curve for MMP8; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [MM0023-7A11] to MMP8 (ab56303) at 5µg/ml and Detector Antibody Rabbit polyclonal to MMP8 - Hinge region (ab38994) at 0.5µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP8 antibody [MM0023-7A11] (ab56303)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MMP8 antibody [MM0023-7A11] (ab56303)](/ps/datasheet/images/56/ab56303/MMP8-Primary-antibodies-ab56303-1.jpg)
ab56303 at 1/200 dilution staining MMP8 in human prostate tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).
Protocols
References for Anti-MMP8 antibody [MM0023-7A11] (ab56303)
This product has been referenced in:
- Radulovic NVet al. Cervical tissue changes in women with miscarriage: a morphological and biochemical investigation. Acta Obstet Gynecol Scand 89:54-64 (2010).Read more (PubMed: 20021266) »
See 1 publication for this product
Publishing research using ab56303? Please let us know so that we can cite the reference in this datasheet
![MMP8 antibody [MM0023-7A11] for WB in Human (56303)](/ps/datasheet/images/56/ab56303/MMP8-Primary-antibodies-ab56303-2.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"