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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Overview
Product name
Anti-MMP8 antibody
See all MMP8 products (12) ...
Description
Rabbit polyclonal to MMP8
Specificity
MMP8 antibody detects endogenous levels of total MMP8 protein.
Tested applications
WB, ELISA, IHC-P, Flow Cyt, IHC-Fr, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from human MMP8
Positive control
Human breast carcinoma tissue; extracts from NIH/3T3 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
Flow Cyt: Use at an assay dependent dilution. (PubMed: 21133852)
IHC-Fr: Use at an assay dependent dilution. (PubMed: 21133852)
IHC-P: Use at an assay dependent concentration.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
Target
Function
Can degrade fibrillar type I, II, and III collagens.
Tissue specificity
Neutrophils.
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization
Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
Target information above from: UniProt accessionP22894
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CLG 1 antibody
- CLG1 antibody
- Collagenase 1 antibody
see all
Database links
- Entrez Gene: 4317 Human
- Entrez Gene: 17394 Mouse
- Entrez Gene: 63849 Rat
- Omim: 120355 Human
- SwissProt: P22894 Human
- SwissProt: O70138 Mouse
- SwissProt: O88766 Rat
- Unigene: 161839 Human
see all
Anti-MMP8 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - MMP8 antibody (ab53017)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MMP8 antibody at 1/50 dilution. Left hand image; untreated tissue, Right hand image; tissue treated with the immunising peptide.
Western blot - MMP8 antibody (ab53017)
All lanes : Anti-MMP8 antibody (ab53017) at 1/500 dilution
Lane 1 : Extracts from NIH/3T3 cells with no immunizing peptide
Lane 2 : Extracts from NIH/3T3 cells with immunizing peptide
Predicted band size : 53 kDa
Observed band size : 53 kDa
Immunocytochemistry/ Immunofluorescence-MMP8 antibody(ab53017)
ICC/IF image of ab63017 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53017, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-MMP8 antibody (ab53017)
This product has been referenced in:
- Clark RTet al. T cell production of matrix metalloproteases and inhibition of parasite clearance by TIMP-1 during chronic Toxoplasma infection in the brain. ASN Neuro : (2010). Flow Cyt, IHC-Fr; Mouse.Read more (PubMed: 21133852) »
See 1 publication for this product
Publishing research using ab53017? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"