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Anti-MMP9 antibody [56-2A4] (ab58803)

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Overview

Product name

Anti-MMP9 antibody [56-2A4]
See all MMP9 products (27) ...

Description

Mouse monoclonal [56-2A4] to MMP9

Specificity

Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP9. Does not react with MMP1, MMP2, MMP3 or MMP13.

Tested applications

IHC-P, WB, IHC-Fr, IHC-Pmore details

Cross reactivity

Reacts with

Rat, Rabbit, Guinea pig, Human

Does not react with

Mouse, Hamster, Cow

Immunogen

Synthetic peptide: LFSGRRLWRFDVKAQMVDP, corresponding to amino acids 626-644 of Human MMP9

LFSGRRLWRF DVKAQMVDP

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Breast carcinoma tissue

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 0.1% BSA, 100mM Sodium phosphate buffer, pH 7.0

Concentration

Concentration information loading...

Purification notes

Is purified.

Clonality

Monoclonal

Clone number

56-2A4

Isotype

IgG1

Research areas

Cardiovascular >> Atherosclerosis >> Thrombosis >> Other
Cancer >> Tumor biomarkers >> Enzymes >> MMPs
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Metalloprotease >> MMPs
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> MMPs
Cancer >> Invasion/microenvironment >> Angiogenesis >> ECM enzymes >> MMPs
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> MMP
Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP

  • Immunohistochemistry (Frozen sections) - MMP9 antibody [56-2A4] (ab58803)Immunohistochemistry (Frozen sections) - MMP9 antibody [56-2A4] (ab58803) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab58803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 78 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.

Tissue specificity

Produced by normal alveolar macrophages and granulocytes.

Involvement in disease

Defects in MMP9 may be a cause of susceptibility to intervertebral disc disease (IDD) [MIM:603932]; also known as lumbar disk herniation (LDH). IDD is one of the most common musculo-skeletal disorders and the predominant cause of low-back pain and unilateral leg pain.
Defects in MMP9 are the cause of metaphyseal anadysplasia type 2 (MANDP2) [MIM:613073]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.

Sequence similarities

Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational
modifications

Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated.

Cellular localization

Secreted > extracellular space > extracellular matrix.

Target information above from: UniProt accessionP14780 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 82 kDa matrix metalloproteinase-9 antibody
  • 92 kDa gelatinase antibody
  • 92 kDa type IV collagenase antibody
  • CLG 4B antibody
  • CLG4B antibody
  • Collagenase Type 4 beta antibody
  • Collagenase type IV 92 KD antibody
  • EC 3.4.24.35 antibody
  • Gelatinase 92 KD antibody
  • Gelatinase B antibody
  • Gelatinase beta antibody
  • GelatinaseB antibody
  • GELB antibody
  • Macrophage gelatinase antibody
  • MANDP2 antibody
  • Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody
  • Matrix Metalloproteinase 9 antibody
  • MMP 9 antibody
  • MMP-9 antibody
  • MMP9 antibody
  • MMP9_HUMAN antibody
  • Type V collagenase antibody
see all

Database links

Anti-MMP9 antibody [56-2A4] images:

  Immunohistochemistry (Frozen sections) - MMP9 antibody [56-2A4] (ab58803)

Immunohistochemistry (Frozen sections) - MMP9 antibody [56-2A4] (ab58803)

ab58803 staining MMP9 in murine rib sections by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 20% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa Fluor®488-conjugated donkey anti-mouse polyclonal (green) was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).

Image courtesy of an anonymous Abreview.

See Abreview

References for Anti-MMP9 antibody [56-2A4] (ab58803)

This product has been referenced in:

  • Speidl WSet al. The complement component C5a is present in human coronary lesions in vivo and induces the expression of MMP-1 and MMP-9 in human macrophages in vitro. FASEB J 25:35-44 (2011). ICC/IF.Read more (PubMed: 20813982) »
  • Fan Met al. The effect of age on the efficacy of human mesenchymal stem cell transplantation after a myocardial infarction. Rejuvenation Res 13:429-38 (2010). WB; Rat.Read more (PubMed: 20583954) »

See all 6 publications for this product

Publishing research using ab58803? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"