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Products:Cardiovascular >> Angiogenesis >> Adhesion / ECM >> Matrix Metalloproteinases >> MMP
Anti-MMP9 antibody - Carboxyterminal end (ab38906)
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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab38906 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - MMP9 antibody - Carboxyterminal end (ab38906)
All lanes : Anti-MMP9 antibody - Carboxyterminal end (ab38906) at 1/1000 dilution
Lane 1 : Human MMP9
Lane 2 : Cell media from human chondrosarcoma (untreated)
Observed band size : 97 kDa (why is the actual band size different from the predicted?)
When used against the reduced protein, this antibody identifies bands at 92 kDa and 88 kDa (the pro form and active form).
Immunocytochemistry/ Immunofluorescence - MMP9 antibody - Carboxyterminal end (ab38906)
ICC/IF image of ab38906 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38906, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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