Western blot abreview for Anti-MMP9 antibody [EP1254]

Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MD-231)
Loading amount
25 µg
Specification
MDA-MD-231
Gel Running Conditions
Non-reduced Denaturing (8% PAGE SDS gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 90 kDa
Username

Abcam user community

Verified customer

Submitted Feb 02 2010

Sign up