Western blot abreview for Anti-MMP9 antibody [EP1254]

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (7.5%)
Sample
Rat Tissue lysate - whole (ovaries)
Specification
ovaries
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TTBS
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: biotinylated anti-rabbit IgG
Host species: Mouse
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Detection

Detection method
ABC
Bands
Specific: 63, 75, 100 kDa Non-specific: 86 kDa
Exposure
5 minute(s) and 0 second(s)
Positive control
ab39308
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Submitted Sep 30 2013

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