Western blot abreview for Anti-MMP9 antibody [EP1254]

Average
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Colon)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
25 µg
Treatment
7.6 mg AOM/kg body weight
Specification
Colon
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% nonfat dry milk
Dilution
1/10000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG (H + L)-HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
Wild type mice that drunk only water
Exposure
30 second(s)
Bands
Specific: 66 kDa Non-specific: 55 kDa
Positive control
Mice with MMP9 overexpressed in the epithelium that drunk only water
Username

Lewins Walter

Verified customer

Submitted Aug 03 2016

Sign up