Western blot abreview for Anti-MMP9 antibody [EP1254]

Excellent
Abreviews
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Application
Western blot
Sample
Human Cell lysate - whole cell (HTB94 chondrosarcoma)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
10 µg
Specification
HTB94 chondrosarcoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% dry milk in PBS
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase

Detection

Detection method
Alkaline phosphatase (BCIP/NBT)
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 92, 83 kDa

Additional data

Additional Notes
Pro MMP-9 was seen in the conditioned media, as established in the literature. In the cell layer, the active form of MMP-9 was more abundant. Clean detection with high sensitivity.
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Submitted Jul 28 2016

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